Lygos, Inc.

University of California

Other

Jeffrey Allen Dietrich

Eric J Steen

Jay D Keasling

Jeffrey L Fortman

Mario Ouellet

Andrew Jonathan Conley

Johan Van Walsem

Yasuo Yoshikuni

Michelle Chia-Yu Chang

Farnaz F Nowroozi

Patricia C Babbitt

Methods and materials related to producing glyceric acid and downstream products are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, methods and materials for producing glycolic acid by direct fermentation from sugars are disclosed.

Recombinant host cells and methods for the production of glyceric acid and downstream products

Methods and materials related to producing isobutyric acid are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, methods and materials for producing isobutyric by direct microbial fermentation from a carbon source are disclosed.

Recombinant host cells and methods for the production of isobutyric acid

Methods for the preparation of bio-based malonic acid and diester derivatives of malonic acid are provided. For example, a dialkyl malonate may be prepared by the steps of (i) separating calcium malonate crystals from a fermentation broth; (ii) obtaining dissolved malonic acid; (iii) crystallizing the dissolved malonic acid; and (iv) performing esterification to obtain the dialkyl malonate. The disclosed methods produce diester derivatives of malonic acid with fewer impurities, which is useful for many industrial processes. The diester derivatives of malonic acid can be purified from existing sources of malonic acid, or from malonic acid made from a renewable carbon source.

Method for preparation of diester derivatives of malonic acid

Methods and materials related to producing D-lactic acid are disclosed. Specifically, isolated synthetic or natural nucleic acids, synthetic or natural polypeptides, host cells, and methods and materials for producing D-lactic acid by direct fermentation from carbon sources are disclosed, along with methods of preparing D-lactic acid polymers.

Recombinant host cells and methods for the production of D-lactic acid

A process for the preparation of bio-based malonic acid and crystalline calcium malonate is provided. The calcium malonate is highly pure and provides a source of malonic acid made from a renewable carbon source rather than existing processes which rely on the use of petroleum-based products. The calcium malonate provides an improved source of malonic acid, which is important to many industrial processes.

Process for purification of malonic acid from fermentation broth

Provided herein are recombinant fungal or bacterial cells comprising a heterologous L-lactate dehydrogenase, and processes of preparing L-lactic acid employing such cells.

Recombinant host cells and methods for the production of L-lactic acid

Systems and methods for the production of malonate in recombinant host cells.

Recombinant host cells for the production of malonate

Compositions and methods for producing malonate in recombinant host cells in order to increase malonate titer, yield, and/or productivity are provided. Recombinant host cells comprising nucleic acids encoding MAE1 transport proteins that increase production of malonate by the host cell and vectors for expressing MAE1 transport proteins that increase production of malonate by the host cell are also disclosed.

The present invention provides for a method of producing one or more fatty acid derived dicarboxylic acids in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived dicarboxylic acids. The invention provides for the biosynthesis of dicarboxylic acid ranging in length from C3 to C26. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism.

Host cells and methods for producing diacid compounds

The invention provides methods and compositions for detecting dicarboxylic acids using a transcription factor biosensor.

Transcription factor-based biosensors for detecting dicarboxylic acids

The present invention provides for a system comprising a BmoR transcription factor, a &#x3c3;-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the &#x3c3;-RNA polymerase.

Transcription factor-based biosensor

The present invention provides methods of designing and generating polypeptide variants that have altered properties compared to a parent polypeptide. The present invention further provides a computer program product for carrying out the design of a variant polypeptide. The present invention further provides nucleic acids encoding enzyme variants, as well as vectors and host cells comprising the nucleic acids. The present invention further provides variant enzymes; methods of producing the variant enzymes; and methods of producing compounds using the enzymes.

Methods of generating protein variants

The present invention provides artemisinic epoxide, and methods of synthesizing artemisinic epoxide in a genetically modified host cell. The present invention further provides methods for producing artemisinin. The present invention further provides variant enzymes that catalyze the oxidation of amorpha-4,11-diene to artemisinic epoxide; nucleic acids encoding the variant enzymes; as well as recombinant vectors and host cells comprising the nucleic acids.

Growing community of inventors

Berkeley, CA, United States of America

Jeffrey Allen Dietrich