Wyeth

Jean Heather Crinean

The present invention provides improved methods for producing a solution containing high molecular weight isolatedcapsular polysaccharides having phosphodiester linkages between saccharide repeat units. In certain methods, COis supplied to a fermentation culture ofbacterial cells that produce capsular polysaccharide serotypes containing phosphodiester linkages between saccharide repeat units. Exemplaryserotypes containing a phosphodiester linkage between saccharide repeat units include serotypes 6A, 6B, 19A, and 19F. Supplying COto the fermentation culture includes adding bicarbonate ions to the fermentation culture, adding carbonate ions to the fermentation culture, adding mixtures of bicarbonate and carbonate ions to the fermentation culture, and overlaying the fermentation culture with CO.

Method for controllingpolysaccharide molecular weight using carbon dioxide

The present invention provides improved methods for producing a solution containing high molecular weight isolated19A capsular polysaccharides. In certain methods, a fermentation culture ofbacterial cells that produce serotype 19A capsular polysaccharides is fermented for less than 6 hours before the bacterial cells are lysed the capsular polysaccharides are harvested. In other methods, COis supplied to the fermentation culture. Supplying COto the fermentation culture includes adding bicarbonate ions to the fermentation culture, adding carbonate ions to the fermentation culture, adding mixtures of bicarbonate and carbonate ions to the fermentation culture, and overlaying the fermentation culture with CO.

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Saint Charles, MO, United States of America

Jean Heather Crinean