Danisco US Inc.

Genencor International, Inc.

E.i. Dupont De Nemours and Company

United States of America, As Represented by the Secretary of Agriculture

The Goodyear & Tire Rubber Company

Dupont Nutrition Biosciences Aps

Dupont Industrial Biosciences Usa, LLC

Danisco US Inc., Genencor Division

Jayarama K Shetty

Michael Jay Pepsin

Gang Duan

Nigel S Dunn-Coleman

Vivek Sharma

Suzanne E Lantz

Oreste J Lantero, Jr

Oreste J Lantero

Ling Hua

Robert DiCosimo

Manoj N Kumar

Richard Ray Bott

Gopal K Chotani

Sung Ho Lee

Zhongmei Tang

Susan Marie Hennessey

Benjamin S Bower

Zheyong Yu

Kevin D Nagy

Toby M Baldwin

Martijn Scheffers

Pieter Van Solingen

Craig E Pilgrim

Birgitta Leuthner

Oliver Kensch

Paulien Neefe-Kruithof

Glenn E Nedwin

Thomas S Green

David B Johnston

Paula Johanna Maria Teunissen

Scott Douglas Power

Andrew Shaw

James T Kellis, Jr

Bo Zhang

Piet Van Solingen

Karl John Sanford

Luis Gustavo Cascao-Pereira

Igor Nikolaev

Kevin B Hicks

Sandra W Ramer

William Albert Cuevas

Robert A Moreau

Anthony R Calabria

Jeff P Pucci

Jing Ge

Sander Van Stigt Thans

Christopher Lee Rife

Bruce A Strohm

Suzanne Breneman

Rodrigo Ramirez

Bradley A Paulson

Kathy Qian

Julius Vadakoot

Mian Li

Brian S Miller

Ying Qian

Erwin F Columbus-Hago

Klaus Schulze Pellengahr

Mai Faurschou Isaksen

Sridevi Vadakoot, Legal Representative

Erwin Columbus Hago

Susan M Fiske

Jacob Flyvholm Cramer

Donald E Ward

William Robert King

Gerhard Konieczny-Janda

Neville Marshall Fish

Peter Edvard Degn

Jaclyn Diana Demartini

Xin Wang

Hongxian Xu

Feng Li

Jacob Andrew Latone

Paulien Kruithof

Kun Zhong

Floor K Kooy

Soeren Dalsgaard

Søren Dalsgaard

Pauline J M Teunissen

Luis G Cascāo-Pereira

Wayne E Henderson

Jalsen Li

Fengi Li

Suzy Breneman

Bart C Koops

Collette M Blake

Douglas Ko

Matt Reboli

Pauline Johanna Maria Teunissen

Martien H Bergsma

Zhen Qian

William King

Eric C Singley

Jr Lantero

The present invention relates to a dry grind ethanol process in which post-distillation backset is subjected to aerobic fermentation to remove components that are inhibitory to an ethanolagen, such as yeast, allowing the utilization of an increased amount of post-distillation backset during the initial preparation of starch-containing substrates for the dry grind ethanol process. Aerobic fermentation of the post-distillation backset allows substantially higher backset recycle, resulting in fresh water savings and increased sustainability.

Method for increasing backset recycle in dry grind alcohol production

Processes of treating grain (e.g., corn), involving milling the grain to produce milled grain wherein the grain germ remains intact in the milled grain, and producing a mixture by mixing the milled grain with water and at least one enzyme selected from the group consisting of protease, alpha amylase, glucoamylase, cell wall degrading enzyme, and mixtures thereof, wherein the pH of the mixture is optionally adjusted to a pH of about 3.5 to about 6.5, and incubating the mixture for about 1 to about 3 hours to produce an incubated mixture.

Processes of treating grain

Fungal glucoamylases from&#x2014;expressed inhost cells (AfGATR) are provided.host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such asalpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed byglucoamylase (AnGA) or native AfGA expressed in. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

host cells expressing a glucoamylase fromand methods of use thereof

A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

Provided herein are variants ofsp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.

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Methods for obtaining corn oil from milled corn germ (e.g., dry milled corn germ), involving adding water, at least one acidic cellulase, at least one acidic protease, and at least one phytase to milled corn germ to obtain corn oil.

Methods for obtaining corn oil from milled corn germ

The present invention relates to polypeptides having glucoamylase activity with improved properties and to compositions comprising these polypeptides suitable for use in the production of a food, beverage (e.g. beer), feed, or biofuel. Also described is an improved and cost-effective process for isolating glucoamylases suitable for large scale protein purification procedures. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.

Polypeptides having glucoamylase activity and method of producing the same

The present invention relates to filamentous fungal host cells and particularlyhost cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch sustrates

Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism, such as isoprene, isoprenoid precursor molecules, and/or isoprenoids. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids

The present disclosure relates to aalpha-amylase (AmyE) or its variants thereof. AmyE or its variants thereof may be used to more efficiently produce fermentable sugars from starch. Also disclosed are a composition comprising a glucoamylase and AmyE or variant thereof and a method of processing starch utilizing the described enzyme composition.

Saccharification enzyme composition and method of saccharification thereof

A dry solids staging fermentation process for producing an end-product, such as ethanol is disclosed said process including an initial fermentation step including combining a first fermentable substrate with one or more starch hydrolyzing enzymes and fermenting organisms in a fermentation vessel and a loading step which includes adding a second fermentable substrate to the fermentation vessel wherein the percent dry solids (% DS) of the fermentation broth increases over time.

Dry solids staging fermentation process

Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

Production of isoprenoids under neutral pH conditions

Growing community of inventors

Pleasanton, CA, United States of America

Jayarama K Shetty