Evonik Degussa Gmbh

Jasmin Gielen

Thomas Haas

Steffen Schaffer

Mirja Wessel

Hans-Georg Joerg Hennemann

Harald Haeger

Markus Poetter

Harald Häger

Frank Erhardt

Wilfried Blümke

Sabine Hafkemeyer

Wilfried Bluemke

Katharina Berse

Marion Schiebelhut

The invention provides a whole cell catalyst which expresses a recombinant &#x3b1;-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the &#x3b1;-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, &#x3c9;-hydroxy fatty acid, &#x3c9;-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, &#x3c9;-hydroxy fatty acid, &#x3c9;-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a &#x3b1;-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

Production of omega-amino fatty acids

A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of &#x3b2;-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the &#x3b2;-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium

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Bochum, Germany

Jasmin Gielen