Martek Biosciences Corporation

Dsm IP Assets B.v.

Jaouad Fichtali

Paul Warren Behrens

Kirk Emil Apt

Jon Milton Hansen

Joseph W Pfeifer, Iii

James Casey Lippmeier

James P Wynn

John M Thompson

Craig A Weaver

Neil Francis Leininger

Jesus Ruben Abril

Micah Needham

Joseph M Kobzeff

Rebecca M Bell

Anand Sundararajan

Henry Linsert, Jr

Sp Janaka Namal Senanayake

Naseer Ahmed

Joseph W Pfeifer

Janaka Namal Senanayake

SP Janaka Namal Senanayake

Joseph W Iii Pfeifer

Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Production of high levels of DHA-containing biomass in microalgae using modified amounts of chloride and potassium

Disclosed are methods of preparing high-quality polyunsaturated fatty acid-containing lipids from a lipid-containing material that include enzymatic treatment of components of the material and/or pressure disruption of the material. Lipid-containing materials include biomass, such as microorganisms. The invention further includes products containing the lipid compositions, such as dietary supplements, food products, pharmaceutical formulations, humanized animal milk, and infant formula.

Method for producing lipids by liberation from biomass

Production of high levels of DHA in microalgae using modified amounts of chloride and potassium

The present invention includes a solid fat composition that includes an oil having saturated fat and a microbial oil having a long chain polyunsaturated fatty acid and an emulsifier. In particular, the solid fat composition can have high levels of long chain polyunsaturated fatty acid and low amounts of emulsifiers. In preferred embodiments, the polyunsaturated oil is an unwinterized microbial oil. The invention also relates to methods for making such compositions and food, nutritional, and pharmaceutical products comprising said compositions. The present invention also includes a microbial oil product prepared by extracting an oil-containing fraction comprising at least one LC-PUFA from a microbial biomass, and treating the fraction by a process of vacuum evaporation, wherein the oil product has not been subject to one or more of a solvent winterization step, a caustic refining process, a chill filtration process, or a bleaching process.

Polyunsaturated fatty acid-containing oil product and uses and production thereof

The present invention includes a palatable, stable composition comprising a biomass hydrolysate emulsion for incorporation, into, or used as, nutritional products, cosmetic products or pharmaceutical products. Preferred sources for biomass are microbial sources, plant sources and animal sources. The present invention also provides methods for making such compositions, specifically, a method for producing a product comprising a nutrient, particularly a long chain polyunsaturated fatty acid, comprising hydrolyzing a biomass comprising the nutrient and emulsifying the hydrolyzed biomass. Such compositions and methods are useful, for example, for increasing intake of nutrients such as omega-3 long chain polyunsaturated fatty acids having 18 or more carbons.

Biomass hydrolysate and uses and production thereof

Methods for discouraging bacterial growth in culture medium

Production of DHA in microalgae in medium having modified amounts of chloride ion and potassium ion

Method to reduce corrosion during fermentation of microalgae

Production of DHA in microalgae in medium having modified amounts of potassium

Methods for the selection of low pH-tolerant, DHA producing microalgae

The present invention relates to a process for preparing a biomass, such as from a microbial fermentation, for an extraction process to separate desired chemicals, nutritional products, bioactive components, proteins, carbohydrates, and lipids, from the biomass. Particularly preferred substances to extract include docosahexaenoic acid, docosapentaenoic acid, and arachidonic acid. The present invention also includes extracting the prepared biomass. Biomasses to be treated in accordance with the methods of the invention include plant, animal, and microbial biomass, particularly a microorganism such asand a fungus such as

Process for preparing materials for extraction

Production of DHA in microalgae in low pH medium

Growing community of inventors

Lexington, KY, United States of America

Jaouad Fichtali