Eli Lilly and Company

Janet K Epp

Brigitte E Schoner

Hansen M Hsiung

JoAnn Hoskins

George L Long

Ramamoorthy Belagaje

The carE gene of Streptomyces thermotolerans has been isolated and used to construct recombinant DNA expression vectors. The carE gene encodes 4'-O-isovaleryl acylase activity important in the biosynthesis of a number of useful antibiotics. The carE gene can be used not only to construct recombinant cells with an increased ability to produce the acylase enzyme but also to construct recombinant cells with the ability to produce novel antibiotic compounds.

Recombinant DNA vectors encoding a 4'-O-isovaleryl acylase derived from

The carG gene of Streptomyces thermotolerans has been isolated and used to construct recombinant DNA expression vectors. The carG gene encodes the activities required for the biosynthesis of the 16-member cyclic lactone of carbomycin. The carG gene can be used not only to construct recombinant cells with an increased ability to produce carbomycin but also to construct recombinant cells with the ability to produce novel antibiotic compounds.

Carbomycin biosynthetic gene, designated carG, for use in streptomyces

The carB gene is a novel carbomycin resistance-conferring gene isolated from Streptomyces thermotolerans and used to construct a number of cloning vectors for use in Streptomyces and related organisms. One such coloning vector, plasmid pOJ159, can be obtained in S. griseofuscus C581 under the accession number NRRL 18090. S. lividans and S. griseofuscus are the preferred hosts when the carB gene is used to select carbomycin-resistant Streptomyces transformants.

Carbomycin resistance-conferring gene, designated carb, for use in

The carA gene is a novel carbomycin resistance-conferring gene isolated from Streptomyces thermotolerans and used to construct a number of cloning vectors for use in Streptomyces and related organisms. One such cloning vector, plasmid pOJ158, can be obtained in S. griseofuscus C581 under the accession number NRRL 18089. S. lividans and S. griseofuscus are the preferred hosts when the carA gene is used to select carbomycin-resistant Streptomyces transformants.

Carbomycin resistance-conferring gene, designated cara, for use in

The present invention comprises improved derivatives of the tryptophan operon useful for expressing fused gene products in E. coli and relate organism. Two of the improved derivatives disclosed are encoded on 0.43 and 0.55 kb EcoRI restriction fragments from plasmids pCZ20 and pLEBGH2 (in strains NRRL B-15881 and NRRL B-15882), respectively. The modified derivatives have been placed on recombinant DNA cloning and expression vectors. A variety of expression vectors have been constructed that drive expression of fused gene products. Two novel gene sequences, encoding insulin-like growth factors I and II, have been expressed with the modified tryptophan operon system.

Growing community of inventors

Indianapolis, IN, United States of America

Janet K Epp