Unilever Patent Holdings B.v.

Lever Brothers Company

Van Den Bergh Foods Co., Division of Conopco, Inc.

Quest International B.v.

Internationale Octrooi Maatschappij 'octropa' Bv

Van Den Bergh Foods Co.

Internationale Otrool Maatschappij 'octropa' B.v.

Jan Maat

Cornelis T Verrips

Adrianus M Ledeboer

Ton Swarthoff

David Thom

Luppo Edens

Cornelis P Hollenberg

Christiaan Visser

Zbigniew A Janowicz

Martinus Roza

Jacob B Visser

Jan Kok

Gerard Venema

Wouter Musters

Johannes M Verbakel

Hein Stam

Robert F M Van Gorcom

Peter J Van De Vonderwoort

Peter J Schaap

Johanna G M Hessing

Josephus Mauritius Van Der Vossen

Johannes Maria A Verbakel

Robert Klok

An isolated DNA with a nucleotide sequence encoding a ripening form of a xylanase fungal origin having bread improving activity. Cells are transformed with this DNA and used to produce the ripening form of xylanase which itself is suitable for use in flour and dough.

Xylanase production

The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, 0in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.

Expression and production of polypeptides using the promoters of the

The claimed invention is drawn to a recombinant plasmid which can replicate in Bacillus subtilis, Escherichia coli, and lactic acid Streptococcus bacteria comprising the replication of origin from Streptococcus cremoris plasmid pWV01 as its origin of replication, in addition to coding marker genes and genes of interest which code for improved fermenting properties.

Plasmids replicatable in Bacillus subtilis, E. coli and lactic acid

The invention relates to recombinant DNA technology for the production of an enzyme having sulfhydryl oxidase ('SOX') activity. This SOX-enzyme can be used where the oxidation of free sulfhydryl groups (thio compounds) to the corresponding disulfides is desirable. SOX enzyme may be used for treatment of bakery products or for removal of off-flavour from milk or beer.

Cloning and expression of DNA encoding a ripening form of a polypeptide

The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.

Regulatory sequences of alcohol oxidase (MOX) and

The invention relates to a detergent composition comprising lipases. By inclusion of a certain immunologically defined class of lipases in a detergent composition which comprises as detergent-active material solely an anionic synthetic detergent, and as builder a water-soluble inorganic or organic builder salt, an improved overall detergency is obtained. The builder salt is typically sodium tripolyphosphate or sodium carbonate, and the lipase is typically obtained from certain Pseudomonas or Chromobacter strains.

Enzymatic detergent composition

A composition is disclosed which comprises cellulase, xylanase, peroxidase, and optionally an oxidase. The composition may be incorporated in flour as an additive to dough for bread or other baked dough products such as puff pastry. Flour compositions comprising a bread improver composition of cellulase, peroxidase and optionally an oxidase, and process for improving baked goods by using same are also shown.

Composition for improving the properties of dough and method of using

The invention relates to structural genes consisting of DNA sequences encoding non-processed and partly processed thaumatin, to the various allelic forms of said non-processed thaumatin and to recombinant DNA's and plasmids comprising said structural genes coding for the various allelic forms of preprothaumatin, and naturally and/or artificially modified preprothaumatin in various stages of its natural processing, and to the use of said recombinant plasmids to transform microorganisms, particularly bacteria in which said genes are expressed.

Structural genes encoding the various allelic and maturation forms of

The invention relates to DNA sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles comprising said DNA sequences and their use in transforming microorganisms.

DNA sequences encoding the various allelic forms of mature thaumatin,

The invention relates to a detergent composition comprising lipases. By inclusion of a certain, immunologically defined class of lipases in a detergent composition which comprises a mixture of an anionic and a nonionic detergent, an improved overall detergency is obtained. Typical suitable lipases are obtained from certain Pseudomonas and Chromobacter strains.

The invention relates to structural genes comprising encoding non-processed and partly processed thaumatin, to the various allelic forms of said non-processed thaumatin and to recombinant DNA's and plasmids comprising said structural genes coding for the various allelic forms of preprothaumatin, and naturally and/or artifically modified preprothaumatin in various stages of its natural processing, and to the use of said recombinant plasmids to transform microorganisms, particularly bacteria in which said genes are expressed.

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Monster, Netherlands

Jan Maat