The United States of America, As Represented by the Secretary, Department of Health and Human Services

Erasmus Universiteit Rotterdam

Jan H J Hoeijmakers

Curtis C Harris

Xin Wei Wang

Gijsbertus Theodoras Johannes Van Der Horst

Wim Vermeulen

Steven Bergink

Mei Yin Ng

A method is provided for determining whether an agent is capable of inducing a DNA lesion in a eukaryotic cell, including exposing the eukaryotic cell to the agent and determining whether an HR23 protein-binding molecule accumulates in the cell, where the HR23 protein-binding molecule is preferably xeroderma pigmentosum group C (XPC), 3-methyladenine DNA glycosylase (MAG), CREB, p53, or a functional part, derivative, and/or analogue thereof. Preferably the cell overexpresses HR23A and/or HR23B protein. A rapid and sensitive test is provided with significant advantages over the Ames test. A method is provided for determining whether an agent is capable of inhibiting a cellular process, the process resulting in accumulation of HR23 protein-binding molecule within a cell. A method for determining whether a cell has an impaired DNA repair system is provided. An impaired DNA repair system is indicative for diseases such as xeroderma pigmentosum, cockayne syndrome, and/or trichothiodystrophy.

Detection methods based on HR23 protein binding molecules

This invention relates to methods of screening for compounds capable of inducing apoptosis in certain tumor cells. The invention also relates to compounds identified by such methods. In addition, the invention relates to methods for the in vitro diagnosis ofand compounds useful in these methods.

Screening assays for compounds that cause apoptosis

This invention relates to methods of screening for compounds capable of inducing apoptosis in certain tumor cells. The invention also relates to compounds identified by such methods. In addition, the invention relates to methods for the in vitro diagnosis of and compounds useful in these methods.

Screening assays for compounds that cause apoptosis and related compounds

This invention relates to methods of screening for compounds capable of inducing apoptosis in certain tumor cells. The invention also relates to compounds identified by such methods. In addition, the invention relates to methods for the in vitro diagnosis of Xeroderma pigmentosum and compounds useful in these methods.

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Zevenhuizen, Netherlands

Jan H J Hoeijmakers