Veridex, Lcc

Stichting Voor De Technische Wetenschappen

Immunivest Corporation

Sequoia-turner Corporation

Biomaterials Research Group Stichting Azl

Jan Greve

Leon W M M Terstappen

Arjan G J Tibbe

John A Verrant

Frank P Modica

Arjan Tibbe

Frank A W Coumans

Dhanesh Gohel

Erik Droog

Leon Terstappen

Clemens Antoni Van Blitterswijk

Frits Frans Maria De Mul

Wiendelt Steenbergen

Tycho M Scholtens

Jan Greve, Legal Representative

Frederik Schreuder

Bernard Gerard De Grooth

Leonardus W Terstappen

Hendrick Klaas Koerten

Tyco M Scholtens

Barth (deceased) De Grooth

Leon W Terstappen

A method for removing excess unbound ferrofluid and imaging immunomagnetically enriched circulating tumor cells is provided. A vessels having a preformed grooves in the viewing surface is optimally designed for cell alignment and imaging. After separating the unbound particles by centrifugation, an externally-applied force is applied to transport magnetically responsive particle-CTC complex toward the transparent collection wall. The grooved inner surface of the viewing face of the chamber provide uniform distribution of the particles for easy imaging. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques as in quantitative analysis of CTC in disease.

Imaging of immunomagnetically enriched rare cells

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

Methods and algorithms for cell enumeration in a low-cost cytometer

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

Methods and algorithms for cell enumeration in low-cost cytometer

Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to automated laser scanning to generate sequential digitized x-y sub-images or partial images of target and non-target objects that are combined to form reconstructed full images, thereby allowing detection, enumeration, differentiation and characterization of imaged objects on the basis of size, morphology and immunophenotype.

Devices and methods to image objects

The invention relates to an optical phantom that is suitable for simulating the optical properties of biological material and to a method of producing said phantom. The phantom is comprised of a matrix of poly(vinyl alcohol) (PVA) and spherical particles whose refractive index differs from that of the PVA. Preferably the PVA has a level of hydrolysis of >98%. Preferably the spherical particles are hollow polystyrene particles. In addition, light-absorbing and light-scattering substances may be added to the matrix.

Optical phantom suitable for stimulating the optical properties of biological material and a method of producing said phantom

Electron microscope with raman spectroscopy

Method and apparatus for analyzing particles based on different depolarizing structure associated with the different particle types. Cells in a flow cytometer are illuminated with a linearly polarized light, producing scattered depolarized light whose intensity range, at a selected measuring angle, and in a suitable discriminating space, is used to analyze different particle types.

Particle discriminator and method

Growing community of inventors

Oldenzaal, Netherlands

Jan Greve