Other

American Registry of Pathology

James W Shih

Jozsef Hepp

Shyh-Ching Lo

Richard Y Wang

Zsolt Lengyel

Rajiv Pande

Ih-Chang Hsu

Barbara Galvan

Ih Chang Hsu

William E Highsmith, Jr

This invention relates generally to nucleic acid-based diagnostic assays for detecting, identifying and/or quantitating target sequences in a sample. More specifically, the invention utilizes sequential cleavage of nucleic acids to achieve target and signal amplification through cycling reactions.

Nucleic acid hybridization assay utilizing tricyclic target and signal amplification

Detection of probe fragment products of basepair mismatch cleavage indicate the presence and sequence of target DNA. Detection of the target is enhanced by amplification through recycling targets by maintaining an assay temperature between the melting point of the target/probe DNA duplex and that of the target/product complex, in the presence of an amplifier comprising ammonium acetate or an amine derivative (for example, diethylamine, piperidine or ammonium carbonate). Cleavage reduces the size of the duplex, and thus lowering its melting point. The amplifier releases the target from the complex, thereby permitting further catalysis of cleavage and effectively amplifying the signal to be detected.

Method of amplifying DNA and RNA mismatch cleavage products

The present invention relates to a diagnostic assay utilizing the lipid associated membrane proteins of mycoplasmas to detect the presence of species-specific mycoplasma antibodies. The sensitive assay is useful for the detection and differentiation of mycoplasma antibodies specific for individual species in biological samples from humans and animals, and exhibits virtually no cross-reactivity between different mycoplasma species.

Mycoplasma diagnostic assay

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Bethesda, MD, United States of America

James W Shih