Eastman-kodak Company

Ortho-clinical Diagnostics, Inc

Johnson & Johnson Clinical Diagnostics, Inc.

Clinical Diagnostic Systems, Inc.

James R Schaeffer

Robert F Winterkorn

Daniel S Daniel

John C Mauck

Harry Wayne Harris

Tsang J Chen

Roy Eugene Snoke

Thomas C Arter

Michael A Schen

Thomas Arter

Thomas R Welter

Richard C Sutton

Harold C Warren

Theodore W Esders

David B LaTart

Ronald H Engebrecht

Louis M Minsk

Karen L Warren

Wayne Weber

Richard E Stevens

Bonny A Harris

A spectrophotometric assay for the detection of acetaminophen in aqueous fluids is carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; and a mild oxidizing agent to oxidize the p-aminophenol so that it couples to a water-soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.

Dry analytical element for acetaminophen assay

A dry analytical element is disclosed which can be used to sensitively and rapidly detect and quantitate protein. The assays are carried out using a dye that reacts with protein and molybdate ion to produce a measurable change in the spectral absorption of the dye. Also disclosed are polymers which stabilize and enhance the accuracy of the assay, and compounds which reduce interference by bicarbonate.

Dry analytical elements for the determination of protein

A spectrophotometric assay for the detection of acetaminophen in aqueous fluids can be carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; a second enzyme, ascorbic acid oxidase, to oxidize the p-aminophenol so that it couples to a water soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.

A method and multilayer analytical element for the determination of catechol and catechol generating substances such as salicylate is described. A series of enzymatic conversions involving tyrosinase is used to convert catechol to o-quinone and the latter to convert a leuco dye to a colored dye.

Method and element for assay of catechol and catechol generating

A multilayer analytical element for quantitatively assaying ethanol comprising a tetrazolium salt, alcohol dehydrogenase, NAD.sup.+, and an electron transfer agent characterized in that the layer comprising the electron transfer agent also includes a polymer having recurring negatively charged groups and the NAD.sup.+ is in a different layer is disclosed.

Ethanol analytical element

A novel 14-crown 4-ether derivative is disclosed for use in a dry analytical element for assaying lithium.

Dry analytical element for lithium assay

A dry multilayer element for assaying salicylate is disclosed.

Dry analytical element for assaying salicylate

Improved fluorescent labels comprise a fluorescent rare earth chelate incorporated into a polymeric particle derived from a loadable latex. The polymer comprises: (a) from about 50 to about 96 weight percent of recurring units derived from a hydrophobic ethylenically unsaturated polymerizable monomer, (b) from about 2 to about 30 weight percent of recurring units derived from a nonionic hydrophilic ethylenically unsaturated polymerizable monomer, and (c) from about 2 to about 20 weight percent of recurring units derived from an anionic ethylenically unsaturated polymerizable monomer containing at least one solubilizing group. The labels of this invention have greatly improved stability in aqueous solutions and do not prematurely agglomerate during storage. They can be attached to any of a variety of physiologically reactive species to provide labeled species, and are particularly useful in specific binding assays.

Latex particles incorporating stabilized fluorescent rare earth labels

Stabilized fluorescent rare earth labels and labeled physiologically

A chromatographic material comprising an inorganic support-polysaccharide particle matrix is described. The matrix comprises an inorganic support having a high surface density of hydroxyl groups and, covalently attached thereto, insoluble particles of a polysaccharide. The free hydroxyl groups of the matrix can be activated, say, by treatment with cyanogen bromide or sodium metaperiodate, to form active sites where ligands can be attached for affinity chromatography.

Composition for chromatography

An analytical element for use in the clinical assay of an analyte contained in a biological liquid sample which is applied from an external source to a substantially planar sample receiving suface of the element. The analytical element comprises a porous layer bearing the aforementioned sample receiving surface; at least one dry reagent material for use in the assay; and one or more associated rupturable pod-like member(s) containing a liquid reagent for the assay, such liquid reagent, upon rupture of the pod-like member(s), being released into the element.

Analytical element for clinical analysis

Catalase is effectively removed from a uricase preparation by amphiphilic chromotography using as the column material cyanogen bromide-activated polysaccharide having a hydrophobic ligand attached thereto. Particularly useful hydrophobic ligands are the alkanediamines.

Purification of uricase

Cholesterol esterase is purified by removal of the separable lipase in an enzyme preparation containing both cholesterol esterase and lipase activities by treatment using a hydrophobic chromatography column comprising carboxylated polysaccharide material having lower alkylamine with less than 8 carbon atoms attached thereto.

Growing community of inventors

Penfield, NY, United States of America

James R Schaeffer