International Minerals & Chemical Corp.

Imc Chemical Group, Inc.

Mallinckrodt Veterinary, Inc.

James R McMullen

Vikram P Mehrotra

Donald R Hahn

A method for producing lysocellin in bead form comprises: (a) cultivating a lysocellin-producing microorganism in an aqueous nutrient-containing fermentation broth under lysocellin-producing conditions; (b) adding a base or inorganic phosphate to the fermentation broth within about the first 24 hours of the beginning of the fermentation in an amount sufficient to ensure that the lysocellin is produced in the form of a cation salt of lysocellin; (c) providing a lipid to the fermentation broth such that the lipid is present in the broth in a sufficient amount to form discrete beads with the lysocellin in the fermentation broth; and (d) separating the beads from the broth.

Preparation of lysocellin beads by fermentation

A high-density fermentation process for high yield production of swine growth hormone by transformant E. coli is described. The process employs transformant strains of E. coli containing an expression vector coding for swine growth hormone under the control of a bacteriophage lambda promoter-operator and an expression vector coding for the cI857 temperature-sensitive repressor protein. In the initial growth period, the level of dissolved oxygen in the fermentation medium is maintained at about 20% to 60% saturation and the temperature of the medium is kept between 26.degree. C. and 30.degree. C. Production of swine growth hormone is then induced by raising the temperature of the medium to about 42.degree. C. The temperature is then reduced to about 40.degree. C. to optimize cell growth for the remainder of the induction period, during which the level of dissolved oxygen in the medium is maintained at about 10% to 40% saturation.

Fermentation process for the high level production of swine growth

A method for microbiologically reducing zearalenone and related compounds comprising cultivating a ketone-reducing microorganism in a nutrient medium and exposing the compound being reduced to the enzyme action of said microorganism.

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Terre Haute, IN, United States of America

James R McMullen