Ucbbiopharma Srl

Ucb Biopharma Sprl

James Heads

Ralph Adams

David Paul Humphreys

Shirley Jane Peters

Alastair David Griffiths Lawson

Sebastian Kelm

The present invention provides a method for generation of multi-domain proteins, more particular antibodies, with an improved native state colloidal stability. The present disclosure provides a method of producing an IgG1 or IgG4 antibody with an improved colloidal stability comprising, in embodiments: calculating for each domain of said IgG1 or IgG4 antibody the total net charge at a given pH; introducing one or more modifications to the amino acid residues of the constant region of said IgG1 or IgG4 antibody to minimize the charge sign difference between the domains, wherein said one or more modification is, or each are, a substitution of a charged amino acid by a polar (non-charged) amino acid; and producing the modified multi-domain protein with improved colloidal stability at the given pH.

Multi-domain proteins with increased native state colloidal stability

The present invention provides an antibody of the class IgG4 comprising at least one heavy chain which comprises a C1 domain and a hinge region, wherein in each heavy chain: a. the inter-chain cysteine at position 127, numbered according to the Kabat numbering system, in the C1 domain is substituted with another amino acid; and b. one or more of the amino acids positioned in the upper hinge region is substituted with cysteine.

Antibodies of the class IGG4

Growing community of inventors

Berkshire, United Kingdom

James Heads