University of Pennsylvania

Leland Stanford Junior University

Other

Agilent Technologies, Inc.

James H Eberwine

Jai-Yoon Sul

Jack D Barchas

Junhyong Kim

Philip G Haydon

Ivan Julian Dmochowski

Russell N Van Gelder

Mark E Von Zastrow

Kevin Miyashiro

Chia-Wen Wu

Fanyi Zeng

Mark I Greene

Ulo Langel

Janet Estee Kacharmina

Hongtao Zhang

Vickas Patel

Teresa L Rapp

Julianne C Griepenburg

Hajime Takano

Jennifer M Singh

Emelia Eiriksdottir

Jaehee Lee

Tae Kyung Kim

Lori Rodgers

Laurence Tecott

Marc Dichter

Ditte Lovatt

Julia Richards

Brittani Ruble

Sean B Yeldell

Max B Kelz

Virginia Man-Yee Lee

John Q Trojanowski

Jinchun Wang

Hong Tao Zhang

Mimi Healy

Kalle Kilk

Jennifer Zielinski

Thomas J Bell

Jason Glanzer

Tiina Peritz (nee Pajunen)

Roger Madison

Stephen Ginsberg

Tracy Kahl McIntosh

Ramesh Raghupathi

Ülo Langel

Dianne M O'Dell

Emelia Eiriksdóttir

Peter Crino

Anup Sharma

Corinne Spencer

Jae-Hee Lee

Stephen Fisher

Jifen Li

Youtao Lu

Tina Peritz

Lindy E Barrett

Kitty Chia-Wen Wu

Chia-Wen Kitty Wu

Kalle c/o Hultberg Kilk

Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.

Transcriptome in vivo analysis (TIVA) and transcriptome in situ analysis (TISA)

Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.

Compositions and methods for genomic DNA and gene expression analysis in single cells

The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.

Transcriptome transfer produces cellular phenotype conversion

The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the transfected cell.

Methods for phototransfecting nucleic acids into live cells

The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.

Methods for transfecting nucleic acid into live cells

The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.

Compositions, methods and kits for real-time nucleic acid analysis in live cells

The invention includes compositions, methods and kits for the in vivo identification of an enzyme that binds to a substrate. The invention comprises, in part, a photoreactive moiety to aid in identification of such an enzyme.

Methods and compositions for in-vivo enzyme capture

Provided are compositions and methods that permit a hybrid nucleic acid-peptide molecule to enter a cell and when specifically activated within the cell, the molecule anneals to endogenous cellular RNA and permits the isolation of the RNA.

Transcriptome in vivo analysis

The invention provides compositions and methods that permit a hybrid nucleic acid molecule to enter a cell and when specifically activated within the cell, the molecule anneals to endogenous cellular RNA and permits the isolation of the RNA.

Methods of detecting transcription

The present invention relates to methods of altering the competence of a dendrite and/or the viability of a neuron by modulating the level of Elk-1 in a dendrite. The present invention also provides methods of altering the ATP levels in a neuron, methods of isolating at least one protein of a mitochondrial permeability transition pore complex, methods of introducing an RNA into a neuron, methods of translating an RNA in a dendrite, methods of monitoring risk of neurodegeneration of a neuron, and methods of treatment for neurodegenerative diseases.

Transcription factors in neuronal dendrites-dendritic protein synthesis and cell death

Growing community of inventors

Philadelphia, PA, United States of America

James H Eberwine