Monsanto Technology LLC

James F Rice

Scott C Johnson

John A Korte

Rebecca A Kelly

Heather M Anderson

Jeanna R Groat

Jennifer R Reyes

Jennifer R Allen

David B Carson

Jennifer Douglas

Scott Johnson

Heather Anderson

The present invention provides a transgenic corn event MON89034, and cells, seeds, and plants comprising DNA diagnostic for the corn event. The invention also provides compositions comprising nucleotide sequences that are diagnostic for said corn event in a sample, methods for detecting the presence of said corn event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said corn event in a sample, growing the seeds of such corn event into corn plants, and breeding to produce corn plants comprising DNA diagnostic for the corn event.

Corn plant and seed corresponding to transgenic event MON89034 and methods for detection and use thereof

PCR-based monitoring of microorganisms in systems for the biological treatment of wastewater. In particular, measuring both the abundance and expression of indicator/effector genes or gene combinations, where expression of an 'effector' gene correlates with the degradative activity of a particular microbial sample, and where the abundance of an 'indicator' gene correlates with the abundance of microbe. Effector gene expression is measured by competitive quantitative RT-PCR, and indicator gene abundance is measured by competitive quantitative PCR. The indicator and effector genes may be the same or different genes. In one embodiment, the indicator and effector gene are sequences from the glyphosate oxidoreductase (gox) gene and the quantitative techniques employed are competitive quantitative PCR and competitive quantitative RT-PCR.

PCR-based monitoring in wastewater biotreatment systems

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St. Charles, MO, United States of America

James F Rice