Cargill Inc.

Elevance Renewable Sciences, Inc.

Evolva Sa

Evolva, Inc.

James C Anderson

Ting Liu Carlson

Dan S Gaspard

Arlene M Fosmer

Richard Nygaard

Kristopher T Mortenson

Robert J Brower, Iii

Nicole Paulson

Belit Flores

Maribeth Rasmussen

Paula M Hicks

Sherry R Kollmann

Mervyn L deSouza

Richard L Pederson

Yann Schrodi

Michael John Tupy

Veronique Douchin

Simon Carlsen

Hiroki Kaido

William H Sperber

Jose Miguel Laplaza

Hans Peter Smits

Manuel Quiros Asensio

Jill Louise Zullo

Ariene M Fosmer

Earl H Wagener

Robert J Iii Brower

Disclosed are methods of releasing or enriching for steviol glycosides produced by yeast. The disclosed methods enhance or improve the release or enrichment of the steviol glycosides.

Heat treatment to produce glycosides

Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. The methods include at least two phases: first and second phases where a glucose-containing feed composition is provided to the medium in different modes of feeding in each phase, such as variable feeding and then constant feeding. The two phase feeding can result in a growth rate that is slower in the second phase than in the first phase, and consequently increased steviol glycoside production rates, reduced fermentation times, and reduced biomass concentrations.

Fermentation methods for producing steviol glycosides with multi-phase feeding

Disclosed arc methods of releasing or enriching for steviol glycosides produced by yeast. The disclosed methods enhance or improve the release or enrichment of the steviol glycosides.

Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. The methods include growing yeast on non-fermentative carbon sources. Other methods include growing yeast on one or more polysaccharides in which saccharification and fermentation of the polysaccharides occurs simultaneously.

Methods for producing steviol glycosides in engineered yeast

Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. In some embodiments, the methods include fermenting with a yeast at a high pH such as of about 5.8 or greater. In some embodiments, the methods can be carried out by first growing the yeast at a lower first pH, and then adjusting the pH to a higher pH.

Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom

Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.

Products and methods for in vivo secretion of monatin

The invention provides surface coating compositions comprising a preservative comprising 9-decenoic acid, salts of 9-decenoic acid, esters of 9-decenoic acid, or combinations thereof. The surface coating compositions may be water-based coatings (e.g., a latex paint) or solvent-based coatings (e.g., a solvent-based paint). The surface coating compositions may be paints, stains, varnishes, concrete coatings, anti-graffiti coatings, and the like. Methods for protecting a surface coating composition from in-can spoilage and protecting a coating of the surface coating composition from deterioration by the action of microorganisms are also described.

Surface coating compositions and methods

Growing community of inventors

Eden Prairie, MN, United States of America

James C Anderson