Cj Cheiljedang Corporation

Cj Corporation

Jae-Ick Sim

Young-Hoon Park

Jeong-Hwan Kim

Yoon-Suk Oh

Jea-Young Chang

Ki-Hoon Oh

Young-Hyeon Kwag

Hyun-Soo Kim

Jin-Ho Lee

Kwang-ho Lee

Hye-Jin Choi

Eun-Sung Koh

Soo-Youn Hwang

Jong-Kwon Han

Jang-Hee Park

Tae-Sun Kang

Won-Sik Lee

Jin-Nam Lee

Provided are a promoter including at least one polynucleotide selected from the group consisting of SEQ ID NOS: 1 to 7, an expression cassette including the same, a vector including the expression cassette, a host cell including the vector, and a method of expressing a gene using the host cell.

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The invention relates to a microorganism, obtained by treatingKCCM 10488 producing 5'-Xanthylic acid as parent strain with UV radiation and mutation Derivatives such as N-methyl-N&#x2032;nitro-N-nitrosoguanidine (NTG), Having a resistance to 5-fluorotryptophan which enhances Biosynthesis of N5-N10-tetrahydrofolate used for transferring Two formyl group during the process of puring biosynthesis, making It possible to accumulate 5&#x2032;xanthylic acid in culture medium at a high Yield and high concentration rate same period of fermentation.

Microorganism producing 5'-xanthylic acid

The invention relates toCJXSP 0201 KCCM 10448 producing 5'-xanthylic acid. More specifically, the invention relates toCJXSP 0201 KCCM 10448 which is a mutant strain ofKCCM 10340 having a resistance to osmotic pressure. In order to obtain mutant strain having enhanced respiratory activity, the present invention adoptedKCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N&#x2032;-nitro-n-nitrosoguanidine (NTG) according to ordinary procedure. Therefore,CJXSP 0201 KCCM 10448 of the present invention makes it possible to overcome growth-standing phase rapidly on early culture and for same period of fermentation, can accumulate 5&#x2032;-xanthylic acid in culture medium at a high yield and concentration rate.

The invention relates toCJXOL 0201 KCCM 10447 producing 5'-xanthylic acid. More specifically, the invention relates toCJXOL 0201 KCCM 10447 which is a mutant strain ofKCCM 10340 having a resistance to oligomycin. In order to obtain mutant strain having enhanced respiratory activity, the present invention adoptedKCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N&#x2032;-nitro-n-nitrosoguanidine(NTG) according to ordinary procedure. Therefore,CJXOL 0201 KCCM 10447 of the present invention makes it possible to increase ATP reproducing activity for same period of fermentation and can accumulate 5&#x2032;xanthylic acid in culture medium at a high yield and concentration rate.

Microorganism producing 5'xanthylic acid

Corynebacterium ammoniagenes KCCM 10340 for producing 5'-xanthylic acid

Growing community of inventors

Icheon-si, South Korea

Jae-Ick Sim