Fermalogic, Inc.

Thermogen Inc.

Other

J Mark Weber

David C Demirjian

Malcolm J Casadaban

Nikos C Pagratis

Veronika Vonstein

Paul E Hessler

Minh B Luu

Andrew C Reeves

Igor Brikun

George L Gaines

Andreas Constantinou

William Henry Cernota

Peter E Larsen

Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.

Soybean-based fermentation media, methods of making and use

A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.

Process of increasing cellular production of biologically active compounds

The present invention relates to a method of improving the strain used for the production of erythromycin through the disruption of the melA gene.

Method for strain improvement of the erythromycin-producing bacterium

We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including ., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kan ). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu Km transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.

Recombinant DNA and methods for producing thermostable enzymes

The instant disclosure teaches transposons and methods of the instant invention for making Protein Fusions by rapid, random shuffling of protein domains to produce novel protein fusions. This system is generally applicable to production of multifunctional chimeric proteins in vivo and in vitro. The methods and constructs of the instant invention can be used to randomly create both carboxy- and amino-terminal protein fusions in vivo. The methods and contructs of the instant invention are useful in the development of a protein domain library, in the construction of multifunctional enzymes, and in the accelerated evolution of new enzymatic activities.

Protein fusion method and constructs

The present invention provides isolated and purified polypeptides that increase antibiotic production, polynucleotides that encode those polypeptides, expression vectors containing those polynucleotides, host cells transformed with those expression vectors, a process of making the polypeptides using those polynucleotides and vectors, and processes using those polypeptides and polynucleotides.

Methods and compositions for enhancing erythromycin production

We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kan.sup.tr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu.sup.- Km.sup.r transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.

Thermophile gene transfer

The present invention provides a process of preparing the isoflavone genistein. Genistein is prepared by fermenting the bacteria Saccharopolyspora erythraea in the presence of a soy-based substrate. A process of recovering genistein from a bacterial fermentation medium using alkaline, organic solvent extraction is also provided.

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Chicago, IL, United States of America

J Mark Weber