Anheuser-busch, Inc.

Irving Ehrenthal

Jagdish Rajpara

Barrett L Scallet

Kenneth K Shieh

Louis F Slapshak

Keith E Miner

This application concerns a method for treating a flocculated homogenate of whole cell glucose isomerase derived from the microorganism Actinoplanes so that the preparation can be dried, preferably after being extruded into cylindrically-shaped particles. The critical step in this method is the addition of a water absorbing smectite filler to the flocculated homogenate prior to drying. The floc also may be frozen and thawed prior to addition of the filler.

Method of treatment of flocculated homogenate of microbial cells

This application concerns a method of preparing high fructose syrups from unrefined high glucose syrups (produced by an acid-enzyme or enzyme-enzyme conversion) by treating the substrate with glucose isomerizing enzyme without the addition of cobalt and/or magnesium salts.

Unrefined glucose syrup as substrate by glucose isomerizing enzyme in

Immobilized glucose isomerase is prepared by mixing 0.5 to 1.5 parts by weight whole microbial cells containing glucose isomerase with one part by weight agar, combining the resultant mixture with an organic solvent, recovering discrete particles of agar gel with the whole microbial cells entrapped therein and drying the agar gel particles. The immobilized glucose isomerase has long half life stability when used in a column in a continuous process to convert glucose to fructose.

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University City, MO, United States of America

Irving Ehrenthal