University of California

Cold Spring Harbor Laboratory

Ira Herskowitz

Jasper D Rine

Bruce W Stillman

Ryuji Kobayashi

Joachim Li

Patricia Laurenson

Francis J McNally

Margit Foss

Masumi Hidaka

Stephen P Bell

Kimberly Gavin

David J Julius

Lindley Blair

Anthony Brake

Janet Kurjan

Jeremy Thorner

George Sprague

Origin of Replication Complex (ORC) genes, nucleic acids which encode ORC proteins and hybridization reagents, probes and primers capable of hybridizing with ORC genes and methods for screening chemical libraries for lead compounds for pharmacological agents useful in the diagnosis or treatment of disease associated undesirable cell growth are provided. An exemplary screen involves forming a mixture comprising a recombinant ORC protein, a natural intracellular ORC protein binding target, and a candidate pharmacological agent; incubating the mixture under conditions whereby, but for the presence of said candidate pharmacological agent, said ORC protein selectively binds said binding target; and detecting the presence or absence of specific binding of said ORC protein to said binding target.

Origin of replication complex genes

Origin of replication complex genes and methods of using the same

A method is provided for expressing the proteases dipeptidyl aminopeptidases A and B which are useful for processing precursor proteins. Genes controlling these proteases may be insdrted into appropriate vectors and transformed into cultures. The proteases may either be utilized to process precursor proteins in vivo or may be extracted from cultures and used to process precursor proteins in vitro.

Cloning and expression of yeast STE13 and Dpp2 genes encoding dipeptidyl

A method for producing secretable and processable biologically useful polypeptides in yeast is provided utilizing vectors including at least a segment of the gene coding for S. cerevisiae alpha-factor.

E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the

Growing community of inventors

Berkeley, CA, United States of America

Ira Herskowitz