Miltenyi Biotec, Gmbh

Miltenyi Biotec B.v. Co., Kg

Ian Johnston

Stefan Miltenyi

Mario Assenmacher

Andrew Kaiser

Volker Huppert

Martin Meyer

Thomas Schaser

Daniel Schäfer

Johann-Christoph Dettmann

Michael Essl

Rita Pfeifer

Mike Essl

Provided herein are chimeric antigen receptors (CARs) that include a) an antigen binding domain specific for an antigen, b) a spacer, c) a transmembrane domain, and d) an intracellular signaling domain, wherein the spacer consists oforC-set Ig-like domain(s) e.g., C-set Ig-like domains of the sialic acid binding Ig-like lectin (Siglec) family. Also provided herein are compositions including a) an immune cell expressing a CAR, wherein the CAR is specific for a tag and b) a tagged polypeptide.

Chimeric antigen receptor with a spacer comprising C2-set Ig-like domains

The present invention provides the use of a packaging cell line for the production of VSV-G pseudotyped retroviral vector particles or virus like particles thereof, wherein said packaging cell line is negative for Low-Density Lipoprotein Receptor (LDLR), optionally said packaging cell line stably expresses VSV-G. A method for producing said VSV-G pseudotyped retroviral vector particles or virus like particles thereof is disclosed as well as said particles obtained by said method.

LDLR negative packaging cell line for the production of VSV-G pseudotyped retroviral vector particles or virus particles thereof

The present invention provides a process for generation of genetically modified T cells, T cell subsets and/or T cell progenitors comprising the steps: a) providing a cell sample comprising T cells, T cell subsets and/or T cell progenitors b) preparation of the cell sample by centrifugation c) magnetic separation of the T cells, T cell subsets and/or T cell progenitors d) activation of the enriched T cells, T cell subsets and/or T cell progenitors using modulatory agents e) genetic modification of the T cells, T cell subsets and/or T cell progenitors f) expansion of the genetically modified T cells, T cell subsets and/or T cell progenitors in a cultivation chamber g) washing of the cultured T cells, T cell subsets and/or T cell progenitors characterized in that all steps are performed in a closed and sterile cell culture system.

Method for automated generation of genetically modified T cells

The invention relates to a process for generation of genetically modified stem cells comprising the steps

Automated generation of genetically modified stem cells

Growing community of inventors

Rosrath, Germany

Ian Johnston