Genex Corporation

Huei-Hsuing Yang

John P Montgomery

Jeffrey C McGuire

Patricia J Vollmer

Jeffrey J Schruben

Malcolm A Finkelman

This invention relates to novel microbial strains which produce phenylalanine ammonia-lyase. These novel microbial strains have the ability to grow on minimal essential media which contain, as substantially the sole carbon source, L-tyrosine. Also disclosed are methods for making phenylalanine ammonia-lyase and methods for making phenylalanine.

Phenylalanine ammonia lyase-producing microbial cells

A method for the enzymatic conversion of t-cinnamic acid and ammonia to L-phenylalanine, using the catalyst, phenylalanine ammonia-lyase, wherein the stability and useful life of the enzyme are improved by conducting the conversion under substantially anaerobic, static conditions.

Method for stabilizing the enzymatic activity of phenylalanine ammonia

A method for the production of the enzyme, phenylalanine ammonia-lyase (PAL), by fermentation, wherein the stability and useful life of the enzyme are improved by maintaining PAL-containing media in substantially anaerobic, static conditions. is useful for catalyzing the conversion of t-cinnamic acid and ammonia to L-phenylalanine.

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Rockville, MD, United States of America

Huei-Hsuing Yang