Development Center for Biotechnology

Hsin Tsai

Chein-Shyong Su

Jin-Tann Chen

Wuen-Hsian Huang

Edward Yi Chang

Hui-Ling Chen

Lung-Shen Lin

Tzann-Shun Hwang

Hsiu-Hui Chen

Szu-Pei Wu

Same-Ting Chen

Hwa-Yi Chen

Huey-Lin Chung

Hsin-Hua Chou

Zen-Jen Chan

The present invention relates to mutant D-amino acid aminotransferase, including nucleic acids encoding mutant D-amino acid aminotransferase. The mutant D-amino acid aminotransferase of the present invention is obtained by the substitution of the glutamate residue at position 13 of wild type D-amino acid aminotransferase from with hydrophobic amino acids. The mutant D-amino acid aminotransferase can be used in the production of D-amino acid and the conversion of glutaryl-7-aminocephalosporanic acid from cephalosporin C. The present invention also includes replica-paper staining method for screening the cells expressing high DAT activity.

D-amino acid aminotransferase for simultaneously producing glutaryl-7-aminocephalosporanic acid and D-amino acid

Disclosed is a mutant aspartase comprising the amino acid sequence of a wild-type E. coli aspartase wherein the amino acid residue at one of positions 25, 123, 421 and 463 is substituted. Also disclosed is a process for preparing the above mutant aspartase.

Mutant aspartase and the preparation thereof

Disclosed are new fluorogenic substrates for assay of angiotensin converting enzyme, a process for preparing them and methods for using them to assay angiotensin converting enzyme and to screen antihypertensive agents which inhibit angiotensin converting enzyme.

Fluorogenic substrates for assay of angiotensin converting enzyme

Disclosed is an automatic computer-controlled bioreactor system for enzymatic synthesis of L-tryptophan which comprises a bioreactor linked to a computer equipped with an on-line indole assay device, in which the computer controls the feeding of indole, pyruvic acid and/or its salt and an ammonium salt into the bioreactor and feedback-controls the reaction in the bioreactor by indole concentration determined by the on-line indole assay device, wherein the feeding of indole and ammonium salt is controlled by a pre-defined profile that agrees with a tryptophanase activity profile and pyruvic acid and/or its salt is added at a predetermined time.

Computer-controlled bioreactor system for enzymatic synthesis of

A process for the preparation of monohydrated sodium phenylpyruvate comprises reacting benzaldehyde with the stoichiometric quantity of hydantoin in the aqueous medium in the presence of a catalytic quantity of a primary or secondary amine at high temperature, treating the reaction mixture with an excess of sodium hydroxide at high temperature, adding sodium chloride to the sodium hydroxide-treated solution, acidifying the so obtained solution with concentrated hydrochloric acid, and washing the formed precipitate with methanol.

Process for the preparation of monohydrated sodium phenylpyruvate

Disclosed is a process for the preparation of N-benzyloxycarbonyl-.alpha.-L-aspartyl-L-phenylalanine methyl ester wherein a protease is used to catalyze the condensation reaction of N-benzyloxycarbonyl-L-aspartic acid with L-phenylalanine methyl ester in a system consisting of ethyl acetate, citrate buffer with the addition of glycerol as a dispersing agent.

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Taipei, Taiwan

Hsin Tsai