Mgi Tech Co., Ltd.

Hongyan Han

Hui Fu Jiang

Wenwei Zhang

Yuan Jiang

Chunyu Geng

Guangying Guo

Guanying Guo

Hui Jiang

The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5'-X-Y-3&#x2032;, and the sequence composition of the downstream random primers is 5&#x2032;-P-Y&#x2032;-X&#x2032;-close-3&#x2032;, wherein Y and Y&#x2032; are random sequences, X is all or part of sequences of a sequencing platform 5&#x2032; end adaptor, X&#x2032; is all or part of sequences of a sequencing platform 3&#x2032; end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken.

Primer for nucleic acid random fragmentation and nucleic acid random fragmentation method

Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5'-end extension primer, wherein the single-stranded 5&#x2032;-end extension primer comprises a sequencing platform adaptor sequence of a 5&#x2032; end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3&#x2032;-end adaptor sequence to the 3&#x2032; end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.

Method and a sequence combination for producing nucleic acid fragments

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Shenzhen, China

Hongyan Han