Ajinomoto Co., Ltd.

Fuji Xerox Co., Ltd.

Hisao Ito

Kazuhiko Matsui

Hiroshi Izui

Osamu Kurahashi

Yoshihiko Hara

Seiko Hirano

Jun Nakamura

Mika Moriya

Yoko Asakura

Mikiko Suga

Tsuyoshi Nakamatsu

Yukiko Mori

Yoshihiko Sakai

Hiroyuki Miyake

Hiroyuki Hotta

Kenichi Hashiguchi

Yuji Joe

Eiji Ono

Yasumoto Shimizu

Yoko Kuwabara

Tsutomu Abe

Masakazu Sugimoto

Mamoru Nobue

Takashi Ozawa

Tomoko Suzuki

Hisashi Yasueda

Kazuhiko Mori

Eiichiro Kimura

Yoshio Nishihara

Teiichi Suzuki

Toshihisa Hamano

Nobuharu Tsujimoto

Tsuyoshi Ohsumi

Ranko Nishi

Sohei Kanno

Atsuko Takai

Mikiko Yamaguchi

Toshihiko Ishiguri

Hiroyuki Kojima

Kazuhiro Sakai

Mario Fuse

Yoshihiro Usuda

Shigeru Nakamori

Yoshio Kawahara

Seigo Makida

Konosuke Sano

Takuji Ueda

Tsuyoshi Inoue

Mutsuo Takenouchi

Takeshi Nakamura

Hajime Sugino

Masayoshi Naruse

Kazuo Nakanishi

Yoshiya Gunji

Hitoshi Enei

Katsuaki Sato

Yuta Nakai

Masako Izui

Takashi Tanaka

Yoko Yamamoto

Yukiko Ono

Naohiro Kadota

Yoshitaka Teratani

Masato Takinami

Saori Kataoka

Seizaburo Shiraga

Hiroko Kishino

Masasi Miyazawa

Hisashi Osada

Noriko Murayama

Yuuta Nakai

Yuri Miyata

Jun Ikeda

Minoru Kida

Akio Nishi

In a method for producing a target substance utilizing a microorganism comprising culturing the microorganism in a medium to produce and cause accumulation of the target substance in the medium and collecting the target substance, there is used, as the microorganism, a mutant strain or a genetic recombinant strain constructed from a parent strain of the microorganism having a respiratory chain pathway of high energy efficiency and a respiratory chain pathway of low energy efficiency as respiratory chain pathways, and having either one or both of the following characteristics:

Method for producing substance utilizing microorganism

A method for producing a target substance by utilizing a microorganism by culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture is described. The microorganism is a mutant recombinant strain in which maltose assimilation is controlled by reducing or eliminating the interaction between IIAprotein of the glucose PTS and a protein involved in non-PTS uptake of maltose.

Method for producing target substance by fermentation

A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

L-glutamic acid-producing microorganism and a method for producing L-glutamic acid

L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genusorand having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

L-glutamic acid-producing bacterium and method for producing L-glutamic acid

A method for producing L-glutamic acid by culturing a coryneform bacterium in which the gluX is inactivated in a medium to produce L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium.

L-glutamic acid-producing bacterium and method for production of L-glutamic acid

A microorganism is cultured in a medium, and is able to produce one or two or more kinds of L-amino acids including L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline and L-arginine, and is modified to increase &#x3b1;-ketoglutarate synthase activity. The L-amino acids are collected from the medium or the cells.

Microorganism producing an amino acid of the L-glutamic acid family and a method for producing the amino acid

A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

Method for producing L-glutamic acid by fermentation accompanied by precipitation

A method for producing L-glutamic acid by culturing a coryneform bacterium which has L-glutamic acid producing ability and which has been modified so that expression of the fasR gene is enhanced in a medium to produce and accumulate L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium or cells.

L-glutamic acid producing bacterium and a method for production of L-glutamic acid

L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

The present invention describes the production of L-tyrosine by culturing in a medium anbacterium which has L-tyrosine-producing ability and which carries a mutant prephenate dehydrogenase which is desensitized to feedback inhibition by L-tyrosine, producing and accumulating L-tyrosine in the medium or in the bacterial cells, and collecting L-tyrosine from the medium or the bacterial cells.

L-tyrosine producing bacterium and a method for producing L-tyrosine

A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.

Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains

An L-amino acid producing bacterium of the Enterobacteriaceae family is described, wherein the bacterium has been modified so as to not produce type I fimbrial adhesin protein is cultured in a medium to produce and excrete said L-amino acid in the medium, and collecting said L-amino acid from the medium.

L-amino acid producing microorganism which has been modified to inactive the fimH gene, and a method for producing I-amino acid

L-tyrosine-producing bacterium and a method for producing L-tyrosine

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Kawasaki, Japan

Hisao Ito