Mitsui Chemicals, Inc.

Idemitsu Kosan Company, Limited

Mitsui Toatsu Chemicals, Incorporated

Hiroshi Uchida

Masaru Honjo

Daisuke Mochizuki

Kazuya Matsumoto

Naokazu Naitoh

Naokazu Naito

Hideki Kobayashi

Masahiko Kuramoto

Keisuke Funaki

Akikazu Nakano

Takashi Sumitomo

Masakazu Suzuki

Nobuhide Ishihara

Toshikazu Ijitsu

Toshihiro Furusawa

Nobumi Kusuhara

Azuma Komeiji

Mihoko Aoki

Tsutomu Sato

Akira Fukuhara

Teruo Ito

Yukio Miyama

A DNA encoding 20K hGH is connected directly to a gene encoding OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, is transformed by the plasmid and cells of the resulting transformant strain are cultured for secretory production of the 20K hGH in the periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the periplasm is low.

Method for secretory production of human growth hormone

A DNA encoding 20K hGH is connected directly to a gene encoding Escherichia coli OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, E. coli is transformed by said plasmid and cells of the resulting E. coli transformant strain are cultured for secretory production of the 20K hGH in the E. coli periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the E. coli periplasm is low.

A stable pharmaceutical preparation containing a human growth hormone having a molecular weight of about 20,000, or a derivative thereof, can be formulated by adding a water soluble heterocyclic compound, such as creatinine, a salt of acetyltryptophane and nicotinamide, to prevent the insolubilization of the human growth hormone or the derivative thereof in an aqueous solution.

Pharmaceutical preparation containing human growth hormone

Efficient secretory production of a recombinant protein in the periplasm can be attained by artificially regulating co-expression of a gene coding for the target recombinant protein and a gene coding for Sec protein involved in protein transport in E. coli cells under conditions in which excessive expression of the Sec protein is suppressed.

Method for secretory production of protein

In producing a recombinant protein in the periplasm of Escherichia coli, the productivity of the target ecombinant protein can be promoted by coexpressing glutathione reductase artificially with the target recombinant protein.

The present invention relates to a styrene-based resin composition comprising a styrene-based polymer having mainly a syndiotactic configuration and a specified antioxidant, a fibrous molding or extrusion produced by spinning a syndiotactic styrene resin composition and a molding produced by shaping and/or spinning, and stretching said resin.

Styrene-based resin composition

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Chiba, Japan

Hiroshi Uchida