Mercian Corporation

Sanraku Incorporated

Zaidan Hojin Biseibutsu Kagaku Kenkyu Kai

Sanraku-ocean Co., Ltd.

Hiroshi Tone

Rokuro Okamoto

Takeo Yoshioka

Hamao Umezawa

Akira Takamatsu

Naoto Kawamura

Kaichiro Kominato

Shin-ichi Hirano

Kazuhiko Okamura

Hiroshi Iguchi

Tomio Takeuchi

Takaaki Aoyagi

Masataka Shirai

Taiji Inui

Toshio Tsuchida

Azuma Watanabe

Akira Arisawa

Naoki Agata

Ryoichi Miyata

Ikuo Kojima

Toshikazu Oki

Masa Hamada

Masaaki Ishizuka

Hajime Morishima

Yasuo Fukagawa

Tsutomu Sawa

Norio Shibamoto

Yoshio Watanabe

Hiroshi Tanaka

Naoki Matsumoto

Hiroyuki Kumagai

Kohki Kiyoshima

Hiroshi Nishida

Hiroyuki Chiba

Tomoyuki Kioka

Hajime Morishama

Masaaki Ishizuki

Toshiyuki Mase

Yasuo Okajima

Reijko Yamamoto

Yutaka Hara

Yasushi Okumura

Koichiro Kominato

Tsutomu Inoue

Isocoumarin derivatives and use thereof in drugs

A DNA fragment or a restriction enzyme-digested DNA fragment, the DNA fragment containing a gene, acyA, encoding a 3-acylation enzyme for macrolide antibiotics, characterized by being derived from a strain belonging to the genus Streptomyces, having a size of about 1.8 kb or about 3.2 kb, and having a DNA base sequence shown in a restriction enzyme map shown in FIG. 1(A) or FIG. 1(B), respectively, in the attached drawing. 3-Acylated macrolide antibiotics can be produced advantageously using macrolide antibiotics-producing bacteria transformed with vector plasmids having inserted therein the DNA fragment.

Genes encoding a 3-acylation enzyme for macrolide antibiotics

A DNA fragment containing acyB gene coding for an enzyme capable of acylating the 4'-position of a macrolide antibiotic, said DNA fragment being derived from a microorganism of the genus Streptomyces, having a size of about 3.1 kb and being characterized by the restriction endonuclease map shown in FIG. 1 , and a DNA restriction fragment resulting from digestion with a restriction endonuclease; a recombinant DNA plasmid containing this DNA fragment; a microorganism transformed with this recombinant plasmid; and a process for producing a 4'-acylated macrolide antibiotic by using the transformant.

DNA coding for enzyme capable of acylating the 4'-position of macrolide

4-Hydroxypyridine derivative represented by the following formula ##STR1## wherein R represents a methyl group or an isopropyl group, and the method for preparing the derivative comprising the step of cultivating a strain belonging to Streptomyces karnatakensis are disclosed. Also disclosed are the pharmaceutical composition comprising the same and a method for treating circulatory diseases comprising the step of administering said compound to a patient.

4-hydroxypyridine derivatives, useful for treating circulatory diseases

Adriamycin derivatives represented by formula (I) ##STR1## wherein R denotes a divalent hydrocarbon group,

Conjugate of adriamycin and cyclodextrin

A process for producing 2-azetidinone derivatives represented by formula ##STR1## wherein R.sup.1 denotes a protecting group of a hydroxyl group,

Process for producing azetidinone derivatives

An angolamycin derivative represented by the following formula ##STR1## wherein X represents an acetyl, methanesulfonyl, methylthio, benzoyl or methoxy group. This compound is useful as a medicament such as antibacterial agent and an animal feed additive.

Angolamycin derivatives

This invention provides a blood typing training kit comprising a red blood cell model and an antiserum model with no use of human blood in experiments for blood agglutination and in learning of blood typing, the red blood cell model being an easily available granule which can be suspended in water such as agarose bead, Sepharose, cellulose, Sephadex, cellulofine, alginate, tamarind polysaccharide, gelatin, xanthan gum, etc. or the saccharide- or protein-bound form thereof and the antiserum model being lectin, boric acid-containing liquid or immunoglobulin G.

Bloodless blood typing training kit

14-Trifluoromethanesulfonyloxydaunomycin represented by the following formula ##STR1## and its salt. This compound is useful as an intermediate for production of adriamycin.

Anthracycline derivatives

Tylosin derivatives represented by the following general formula (I): ##STR1## [wherein R denotes a hydrogen atom, acetyl, propionyl or a radical of Si(R.sup.2).sub.3 (in which R.sup.2 is a lower alkyl group), and R.sup.1 stands for a hydrogen atom or a radical of Si(R.sup.2 .sub.3 ] are useful as intermediates for the synthesis of 4'-acyl derivatives of tylosin because, after introduction of any desired acyl group at the 4'-hydroxyl group, the silyl protective group can be readily removed without liberation of the acyl group. This invention also provides processes for producing the tylosin derivatives. More specifically, it provides a melthod of selectively protecting 2'- and 4''-hydroxyl groups of tylosin (those of higher reactivity among the hydroxyl groups involved); a method of selectively protecting only the 4''-hydroxyl group of tylosin; a method of selectively protecting the 3- and 4''-hydroxyl groups of 2'-O-acyltylosin; and a method of protecting 3-, 2'- and 4''-hydroxyl groups of tylosin.

Tylosin derivatives and processes for producing the same

A novel physiologically active peptide Val-X-Ala-X, in which X is 4-amino-3-hydroxy-6-methylheptanoic acid, which is prepared from R-Val-Val-X-Ala-X by the action of a microbial enzyme, N-acyl derivatives thereof which are produced by acylating said new peptide, and the processes for producing thereof and the microbial enzyme are disclosed.

Novel physiologically active peptide and its n-acyl derivatives and

A novel physiologically active peptide Val--X--Ala--X, in which X is 4-amino-3-hydroxy-6-methylheptanoic acid, which is prepared from R--Val--Val--X--Ala--X by the action of a microbial enzyme, N-acyl derivatives thereof which are produced by acylating said new peptide, and the processes for producing thereof and the microbial enzyme are disclosed.

Processes for producing physiologically active peptide and its N-acyl

A new antitumor agent named macracidmycin, which is a macromolecular peptide and inhibits the growth of various tumor cells and experimental animal tumors such as Ehrlich ascites carcinoma and Sarcoma 180 is produced by the fermentation of a microorganism belonging to the genus Streptomyces which has been designated Streptomyces atrofaciens (M590-G2 and A.T.C.C. 31104); it is recovered from the broth by conventional methods for recovering antibiotics.

Antitumor antibiotic macracidmycin

Growing community of inventors

Yokohama, Japan

Hiroshi Tone