Shionogi & Company Limited

Shionogi Sieyaku Kabushiki Kaisha

Otsuka Pharmaceutical Company, Limited

Sanwa Kagaku Kenkyusho Co., Ltd.

Shionogi Seiyaku Kabushiki Kaisha

Hiroo Imura

Kazuwa Nakao

Shigetada Nakanishi

Masayasu Kurono

Kiichi Sawai

Takahiko Mitani

Yoshikatsu Hirai

Haruo Takahashi

Kenichi Tanaka

Yutaka Seino

Junichi Fukata

DNA encoding an endothelin receptor shown in SEQ ID NO: 1 or 2 in the Sequence Listing is isolated from cDNA which is prepared from poly(A)RNA derived from a human placenta. In addition, an expression vector containing the DNA and a transformant containing the expression vector are obtained. An endothelin receptor is obtained by culturing this transformant. A receptor shown in SEQ ID NO: 1 is an ET-receptor which has a high affinity for endothelins 1 and 2, especially for the endothelin 1. A receptor shown in SEQ ID NO: 2 is an ET-receptor which has an affinity for endothelins 1, 2 and 3 with no selectivity.

Human endothelin receptor

DNA encoding an endothelin receptor shown in SEQ ID NO: 1 or 2 in the Sequence Listing is isolated from cDNA which is prepared from poly(A) RNA derived from a human placenta. In addition, an expression vector containing the DNA and a transformant containing the expression vector are obtained. An endothelin receptor is obtained by culturing this transformant. A receptor shown in SEQ ID NO: 1 is an ET -receptor which has a high affinity for endothelins 1 and 2, especially for the endothelin 1. A receptor shown in SEQ ID NO: 2 is an ET -receptor which has an affinity for endothelins 1, 2 and 3 with no selectivity.

DNA encoding an endothelin receptor shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. In the Sequence Listing is isolated from cDNA which is prepared from poly(A),RNA derived from a human placenta. In addition, an expression vector containing the DNA and a transformant containing the expression vector are obtained. An endothelin receptor is obtained by culturing this transformant. A receptor shown in SEQ ID NO: 1 and SEQ ID NO: 2 is an ET,-receptor which has a high affinity for endothelins 1 and 2, especially for the endothelin 1. A receptor shown in SEQ ID NO: 3 and SEQ ID NO: 4 is an ET,-receptor which has an affinity for endothelins 1, 2 and 3 with no selectivity.

A method for testing the inducibility of the hypothalamus to effect the secretion of andrenocorticotropic hormone comprising the steps of:

Method for stimulating the secretion of ACTH by administration of IL-1B

A monoclonal antibody which recognizes the N-terminal of .alpha.-atrial natriuretic polypeptide (.alpha.-ANP). A hybridoma producing said monoclonal antibody and a process for immunoassay of .alpha.-ANP are also provided.

Monoclonal antibody recognizing atrial natriuretic polypeptide

A monoclonal antibody which recognizes the N-terminal of gamma-atrial natriuretic polypeptide (.gamma.-ANP). A hybridoma producing said monoclonal antibody and a process for immunoassay of .gamma.-ANP are also provided.

Monoclonal antibody recognizing gamma atrial natriuretic polypeptide

A cloned single-strand DNA comprising nucleotide sequence which encodes human motilin precursor, a cloned double-strand DNA consisting of the single-strand DNA and its complementary single-strand DNA, a fragment of the single- or double-strand DNA, a plasmid, in which the double-strand DNA or its fragment is integrated, as well as a process for the preparation of the single- or double-strand DNA.

Growing community of inventors

Kyoto, Japan

Hiroo Imura