Shimadzu Corporation

Mitsui Chemicals, Inc.

Hiroki Kuyama

Norikazu Ueyama

Shigemi Norioka

Eiji Ando

Minoru Yamaguchi

Takashi Nakazawa

Taka-aki Okamura

Osamu Nishimura

Eiichi Ozeki

Masahide Tanaka

Kenji Takeda

Motosada Kiri

Junichi Kita

Masaaki Oota

Takeshi Ishitoku

Syotaro Oka

Akara Hashimoto

Naoya Sakamoto

Takashi Obama

Jiro Ishihara

Kazuhiro Sonomura

According to the present invention, a matrix reagent prepared by adding methylenediphosphonic acid (MDPNA) having two phosphonic acid groups as an additive to a matrix of 2,5-dihydroxybenzoic acid (DHBA) is used for the preparation of a sample. By using the sample according to the present invention, it is possible to achieve a higher peak intensity of phosphopeptide as compared with a sample in which DHBA is solely used as a matrix without using an additive, or a sample in which phosphoric acid (PA) is used as an additive in addition to DHBA. Further, use of the sample according to the present invention enables the detection of peptides that cannot be detected in a case of the phosphoric acid-added sample.

Phosphopeptide analysis method

The present invention provides a method for effectively introducing sulfonic acid groups into the N-terminus of a protein or a peptide. The method comprises a modification step to react a N-terminus in a protein or peptide with a compound A which includes disulfide group and a cleavage step to cleave a disulfide bond of the disulfide group to convert into a sulfonic acid group. The present invention also provides a method of analyzing proteins or peptides easily and effectively on mass spectrometry and an intermediate that can be used to effectively derivatize proteins or peptides as sulfonic acid derivatives.

Methods for derivatizing protein or peptide with sulfonic acid groups

A simple and low-cost method of selectively modifying the C-terminal of a protein or peptide is provided. A method of modifying the C-terminal of a protein or peptide comprises forming an intramolecular oxazolone ring at the C-terminal of the protein or peptide that requires C-terminal modification, and then performing a ring-opening of the oxazolone ring to produce a protein or peptide with a modified C-terminal. Preferred forms include a method in which by reacting the oxazolone ring with a compound containing a nucleophilic group to effect an oxazolone ring-opening, a protein or peptide is produced in which the C-terminal is modified with the compound containing the nucleophilic group, as well as a method in which by reacting the oxazolone ring with an active esterifying agent to effect a ring-opening, the oxazolone is converted to an active ester, which by subsequent reaction with a compound containing a nucleophilic group, produces a protein or peptide in which the C-terminal has been modified with the compound containing the nucleophilic group.

Method for modifying protein or peptide C-terminal

A crosslinked polycarbonate is obtained by polycondensation of diol (A), trivalent or more polyhydric alcohol (B) whose arbitrary two hydroxy groups are not in the positional relationship of 1,2-disubstitution or 1,3-disubstitution, and carbonyl component (C) such as carbonic acid diesters. In the polycondensation step, no side reaction does not occur. This crosslinked polycarbonate is used as a modifier for polylactic acids. The brittleness of the polylactic acid is improved while maintaining mechanical strength, thermostability, and transparency.

Crosslinked polycarbonate and polylactic acid composition containing the

The polylactic acid-based resin composition includes (a) (i) 100 parts by weight of a poly-L-lactic acid; or (ii) 100 parts by weight of a poly-DL-lactic acid comprising L-lactic acid and D-lactic acid as its constituting units; and (b) 5 to 120 parts by weight of at least one of low-molecular lactic acid derivatives. The molded product is produced by the steps of 1) melt-kneading (a) one of the above components (i) or (ii) and the component (b); and 2) subjecting the melt-kneaded product obtained in step 1) to an injection molding, to give a desired molded product.

Polylactic acid-based resin composition and use therefor

Thermal detectors comprising at least one pair of membrane electrodes formed on a base material (such a pyroelectric, semiconductive and insulating materials), wherein at least one member of said pair of electrodes is covered with a black membrane of precious metal formed by the electrolytic process, are highly sensitive because of the excellent heat absorption efficiency, with little change in sensitivity over a wide wavelength range.

Thermal detectors and process for manufacturing the same

Growing community of inventors

Kyoto, Japan

Hiroki Kuyama