Showa Denko K.k.

Hokkaido University

Sds Biotech K.k.

Hirobumi Aoki

Harumi Kamachi

Motoaki Kamachi

Yohei Kurata

Yuzuru Kokido

Kenji Tajima

Tomoki Erata

Tadashi Yoneda

Keijitsu Tanaka

Yoko Hashimoto

Tsuyoshi Katoh

Seiichi Taguchi

Takashi Echigo

Ken'ichiro Matsumoto

Junji Sasuga

Hirotsugu Nakanishi

Kazufumi Yazaki

The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.

Separation/analysis method for mixture of oligonucleotides

A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.

Manufacturing method for 1,4-butanediol, microbe, and gene

The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene.

Production method for organic acid using CoA-transferase

A skin agent for external use and a cosmetic agent are provided, by transdermal administration of which expected actions and effects of ubiquinone derivatives, salts thereof, ubiquinones and ubiquinols are effectively obtained. The skin agent for external use includes a ubiquinone derivative or a salt thereof as an active ingredient. The ubiquinone derivative is represented by the formula (1): wherein Rand Rare each a hydrogen atom or a phosphoric group, at least one of Rand Ris a phosphoric group, and n is an integer in the range of 1 to 9.

Skin agent for external use and cosmetic agent including ubiquinone derivative or salt thereof and method using the same

The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polymers such as polyester.

Method for acyltransferase reaction using acyl coenzyme A

Hydroxycitric acid derivatives and salts thereof are provided which are useful as active ingredients of skin external preparations and cosmetics. Also provided are processes for production of the hydroxycitric acid derivatives, and skin external preparations and cosmetics containing the hydroxycitric acid derivatives. Specific hydroxycitric acid derivatives or salts thereof are produced wherein at least one of the hydroxyl groups of hydroxycitric acid or least one of the hydroxyl groups and at least one carboxyl group of hydroxycitric acid are modified to linkage moieties breakable by biological enzyme reaction. The hydroxycitric acid derivatives or salts thereof are added in skin external preparations and cosmetics.

Hydroxycitric acid derivatives and skin external preparations containing the same

To provide novel carnitine derivatives and salts thereof that are resistant to hydrolysis in the presence of aqueous media, and also provide external skin preparations and cosmetics that are excellent in storage stability, product life, skin affinity and percutaneous absorption properties. In particular, to provide novel &#x3b1;-branched acyl carnitine derivatives and salts thereof, external skin preparations and cosmetics comprising specific &#x3b1;-branched acyl carnitine derivatives and/or salts thereof.

Carnitine derivative, salt thereof, external skin preparation and cosmetic material

The invention relates to a selection method of anmutant strain which expresses an exogenous gene at a high level, wherein stress resistance such as hydrogen peroxide decomposition activity is used as an index, themutant strain selected thereby and production methods of enzyme using the strain and of useful compounds like amino acids (especially L-amino acid) and the like using the strain. According to the selection method of the invention, anmutant strain where gene expression amount does not decrease with passage can be obtained, and a compound using a plant-derived ammonia lyase can be efficiently produced.

[object Object]

The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polylmers such as polyester.

The present invention relates to a novelbacterium and to a process of hydrolyzing a cyano group of a nitrile compound using a novelbacterium to produce the corresponding carboxylic acid. The present invention also relates to a process of producing carboxylic acids, in particular cyano carboxylic acids using a transformant transformed with a plasmid containing a nitrilase gene, a nitrile hydratase gene and an amidase gene derived frombacteria capable of exhibiting particularly excellent position selectivity for the cyano group of aromatic polynitrile compounds, to such a transformant, such a plasmid, to such genes, to a process of producing an enzyme using the transformant, and to enzymes obtained by the process. The carboxylic acids, in particular cyano carboxylic acids obtained by the present invention are useful as starting materials for the synthesis of drugs, agrochemicals, dyestuff and other chemicals.

A method for treating wood, characterized by impregnating wood with a metal-containing treating agent containing lignin and/or lignin derivatives and a metal, a metal compound, and/or a metal ion and oxidizing and/or macromolecularizing of the lignin and/or lignin derivatives in the wood to fix the metal component in the wood, and wood and woody materials obtained by the treating method. According to the method of the present invention, while effectively utilizing lignins, lignosulfonic acids, or lignosulfonic acid salts, leaching of the metal component can be suppressed to a low level to thereby retain the effects due to the incorporation of the metal components for a prolonged period.

Growing community of inventors

Chiba, Japan

Hirobumi Aoki