Genpharm International, Inc.

Hein Te Riele

Anton J Berns

Els Robanus Maandag

The present invention provides novel methods for modifying the genome of an animal cell which typically comprise the steps of: constructing a DNA molecule in which desired sequence modifications are contained in a segment of DNA (a &ldquo;targeting DNA&rdquo;) that is substantially isogenic with a DNA in the cell genome (a &ldquo;target DNA&rdquo;); introducing the targeting DNA construct into the cell (e.g., by microinjection, electroporation, transfection, or calcium phosphate precipitation); and selecting cells in which the desired sequence modifications have been introduced into the genome via homologous recombination.

High efficiency gene targeting in mouse embryonic stem cells

The present invention provides novel methods for modifying the genome of an animal cell which typically comprise the steps of: constructing a DNA molecule in which desired sequence modifications are contained in a segment of DNA (a 'targeting DNA') that is substantially isogenic with a DNA in the cell genome (a.'target DNA'); introducing the targeting DNA construct into the cell (e.g., by microinjection, electroporation, transfection, or calcium phosphate precipitation); and selecting cells in which the desired sequence modifications have been introduced into the genome via homologous recombination.

Growing community of inventors

Amsterdam, Netherlands

Hein Te Riele