Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Danstar Ferment Ag

Enchi Corporation

Universiteit Stellenbosch

Heidi H Hau

John E McBride

Erin Wiswall

Charles F Rice

Emily Stonehouse

Haowen Xu

Indraneel Shikhare

Aaron Argyros

Trisha Barrett

Nicky Caiazza

Allan C Froehlich

Anne K Warner

Mark Mellon

Ryan Skinner

Riaan Den Haan

Shital A Tripathi

Chhayal V Gandhi

Kristen M Deleault

John S Bardsley

Frank Agbogbo

Kevin S Wenger

Alan Belcher

Jennifer Gosselin

Yin-Ying Chiu

Abigail Foster

Vineet B Rajgarhia

Elena E Brevnova

John E Mcbride

Arthur J Shaw, Iv

Naomi Thorngren

Elena Brevnova

Chris Herring

Abigail S Foster

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

Expression of enzymes in yeast for lignocellulose derived oligomer CBP

Thermophilic gram-positive anaerobic host cells, for example(''), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2. In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, ahost cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

Heterologous biomass degrading enzyme expression in thermoanaerobacterium saccharolyticum

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Saint Paul, MN, United States of America

Heidi H Hau