Boehringer Mannheim Gmbh

Hans Moellering

Gunther Schumacher

Georg-Burkhard Kresse

The invention concerns a new 6-phosphogluconolactonase which has an enzyme activity of at least 90% with respect to the initial activity after about 20 hours at pH 7.0 and 20.degree. C. In addition a process is described for the production of the enzyme from Leuconostoc. The enzyme is preferably suitable for the determination of ATP or reaction partners which can be converted into 6-phosphogluconolactone.

Process for the production and use of a stable 6-phosphogluconolactonase

The present invention provides a pyruvate oxidase which decarboxylates pyruvate to form inter alia hydrogen peroxide and is active without the addition of FAD, thiamine pyrophosphate and divalent metal ions. The amino acid sequence of the enzyme changes at least one proline in position 178 and alanine in position 458 to a different amino acid. The present invention also provides a process for the preparation of this pyruvate oxidase and methods of use thereof.

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Tutzing, Germany

Hans Moellering