Bgi Shenzhen

Bgi-shenzhen Co., Limited

Mgi Tegh Go., Ltd.

Hanjie Shen

Yuan Jiang

Wenwei Zhang

Xia Zhao

Tao Zeng

Yongwei Zhang

Yang Xi

Jianxun Lin

Qiaoling Li

Guoli Zhu

Pengjuan Liu

Radoje Drmanac

A nanopore sequencing method, including: in a nanopore sequencing device, performing rolling circle amplification by using a single-strand ring or double-strand ring of nucleic acid as a template to produce an amplified single strand; under the action of an electric field, introducing the amplified single strand into nanopores of the nanopore sequencing device, so that the amplified single strand passes through the nanopores and generates an electrical signal for each base. By determining electrical signal differences caused by different bases, a base sequence on a template nucleic acid is measured.

Nanopore sequencing method

Provided are a hooked probe, a method for ligating a nucleic acid and a method for constructing a sequencing library. The hooked probe comprises a target specific area and a hooked area ligated thereto; the target specific area comprises a sequence complementarily paired with at least part of the single chain of the nucleic acid fragment to be ligated; the hooked area comprises a sequence unpaired with the nucleic acid fragment; the end of the hooked area is a ligatable end; and the ligatable end can ligate the end of the single chain of the nucleic acid fragment.

Hooked probe, method for ligating nucleic acid and method for constructing sequencing library

Growing community of inventors

Guangdong, China

Hanjie Shen