Biocon Limited

Gokul Jothiraman

Sanjay Tiwari

Kedarnath Nanjund Sastry

Nagaraj Govindappa

Partha Hazra

Mukesh Babuappa Patale

Gourav Awasthi

Nandini Nataraj

Arun Chandavarkar

The present disclosure relates to a comprehensive model for expression of recombinant peptides by. The model uses an easily controllable variable called 'critical nutrient ratio' for obtaining a right balance between product synthesis and it's degradation during the fermentation process. The extra cellular concentration of precursor could be increased by about 10 folds and the degradation constants could be reduced by about 10-20 folds for intracellular and extracellular cases respectively by controlling critical nutrient ratio and addition of soya flour hydrolysate and EDTA.

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The present invention relates to novel Prolipase-Bovine trypsinogen (PLBTR) fusion proteins, the genes encoding them, and the production and uses thereof. More specifically, the present invention relates to methods of producing in optimal quantities PLBTR fusion proteins which comprise a heterologous polypeptide which is normally susceptible to autocatalytic activity. More particularly, the present invention relates to fusion proteins which comprise an heterologous polypeptide, such as a serine protease, fused to a lipase signal sequence, which can be expressed by recombinant host cells in desired amounts. The present invention further relates to polynucleotides encoding such fusion proteins, to expression vectors for expression of such fusion proteins, to host cells transformed with such polynucleotides/vectors, and to methods of generating such fusion proteins.

Fusion proteins and method of expression thereof

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Nadu, India

Gokul Jothiraman