Eniricerche S.p.a.

Giovanna Carpani

Guido Grandi

Renata Grifantini

Giuliano Galli

The present invention relates to thermostable mutants of D-N-.alpha.-carbamoylase and means and methods for their preparation. The thermostable mutants have an unaltered or improved activity with respect to the wild-type enzyme and are particularly useful in the preparation of D-.alpha.-amino acids.

Thermostable Mutants of D-N-.alpha.-carbamoylase

The present invention relates to thermostable mutants of Agrobacterium radiobacter D-N-.alpha.-carbamoylase and means and methods for their preparation. The thermostable mutants have an unaltered or improved activity with respect to the wild-type enzyme and are particularly useful in the preparation of D-.alpha.-amino acids.

Thermostable mutants of D-N-.alpha.-carbamoylase

In an improved process for the preparation of D-.alpha.-amino acids by the stereoselective conversion of racemic mixtures of 5-substituted hydantoins with an enzymatic system produced by a microorganism, the improvement consists in the fact that a microorganism is used which is transformed with the plasmid pSM700 cultivated at a temperature of between 20.degree. C. and 28.degree. C. The use of this microorganism allows an improvement in the expression of the enzymatic system and an increase in the conversion rate of the racemic hydantoin to D-.alpha.-amino acid.

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Sergnano, Italy

Giovanna Carpani