National Research Council of Canada

G Dennis Sprott

Girishchandra Babubhai Patel

Boby Makabi-Panzu

Dennis M Whitfield

Lakshmi Krishnan

Irena Ekiel

Douglas L Tolson

Subash Sad

Christian G Choquet

A synthetic charged glycolipid is described comprising a sulfated saccharide group covalently linked to the tree sn-1 hydroxyl group of the glycerol backbone of an archaeal core lipid via a beta linkage. The synthetic charged glycolipids include compounds of formula I wherein n is 0 or 1; R is hydrogen or hydroxyl; and Y is hydrogen or a sulfate group, at least one Y being a sulfate group; and including pharmaceutically acceptable salts thereof. The sulfated glycolipid produces stable archaeosomes at a mol % ratio of from 100:0 to 30:70 (sulfated glycolipid:uncharged glycolipid) and which induce a protective immune response, including CD8and CD4T cell responses. Archaeosomes comprising the sulfated glycolipids described have desirable adjuvant properties, particularly when mixed with uncharged glycolipid at a mol % ratio of about 50:50. 

Sulfated-glycolipids as adjuvants for vaccines

Novel archaeosome compositions and their use in vaccine formulations as adjuvants and/or delivery systems, to enhance the immune response to immunogens in an animal such as a human, are described. Another aspect relates to the use of these archaeosomes to enhance the delivery of compounds such as pharmaceuticals to specific cell types and tissues in animals and other life forms, via various routes of administration such as subcutaneous, intramuscular, and oral. The efficacy of the archaeosomes and also of conventional liposomes can be further improved in these applications, by incorporation of coenzyme Q and/or polyethyleneglycol-lipid conjugate into liposomes made from these archaeosomes.

Archaesomes, archaeosomes containing coenzyme Q10 and other types of liposomes containing coenzyme Q10 adjuvants and as delivery vehicles

Archaeosomes, archaeosomes containing coenzyme Q.sub.10, and other types

Formation of stable liposomes from lipid extracts of archaeobacteria

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Ottawa, Canada

G Dennis Sprott