Amoco Corporation

Friedhelm L Brinkhaus

James G Yarger

Indrani Mukharji

Rodney L Ausich

Huei-Che B Yen

John H Proffitt

William H Eschenfeldt

Randal Hauptmann

Jami English

DNA segments encoding the Erwinia herbicola enzymes geranylgeranyl pyrophosphate (GGPP) synthase, phytoene synthase, phytoene dehydrogenase-4H, lycopene cyclase, beta-carotene hydroxylase, and zeaxanthin glycosylase, DNA variants and analogs thereof encoding an enzyme exhibiting substantially the same biological activity, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes, zeaxanthin and zeaxanthin diglucoside by recombinant DNA technology in transformed host organisms are disclosed.

Biosynthesis of zeaxanthin and glycosylated zeaxanthin in genetically

DNA segments encoding the Erwinia herbicola enzymes geranylgeranyl pyrophosphate (GGPP) synthase, phytoene synthase, phytoene dehydrogenase-4H and lycopene cyclase, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes and beta-carotene by recombinant DNA technology in transformed host organisms are disclosed.

Beta-carotene biosynthesis in genetically engineered hosts

A transgenic higher plant, seed containing the transgene, and methods of providing enhanced carotenoid accumulation are disclosed. The transgenic higher plant has a genomic structural gene that encodes a chimeric polypeptide conjugate and over accumulates a colored native carotenoid in a preselected storage organ relative to the accumulation in a non-transgenic plant of the same type. Expression of the chimeric polypeptide is driven by a promoter operatively linked to that structural gene that provides storage organ-enhanced expression. The chimeric polypeptide has an N-terminal plastid transit peptide portion whose C-terminus is linked to the N-terminus of a non-higher plant phytoene synthase enzyme.

Enhanced carotenoid accumulation in storage organs of genetically

DNA segments encoding the Erwinia herbicola enzymes geranylgeranyl pyrophosphate (GGPP) synthase and phytoene synthase, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes and phytoene by recombinant DNA technology in transformed host organisms are disclosed.

Phytoene biosynthesis in genetically engineered hosts

DNA segments encoding the Erwinia enzymes geranylgeranyl pyrophosphate (GGPP) synthase, phytoene synthase and phytoene dehydrogenase-4H, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes and lycopene by recombinant DNA technology in transformed host organisms are disclosed.

Growing community of inventors

Lisle, IL, United States of America

Friedhelm L Brinkhaus