Biomerieux, Inc.

Commissariat a L'energie Atomique Et Aux Energies Alternatives

Biomerieux Sa

Centre National De La Recherche Scientifique-Cnrs

Université Jean Monnet Saint-Étienne

Centre National De La Recherche Scientifique

Frédéric Pinston

Laurent Drazek

Corine Fulchiron

Hervé Rostaing

Dominique Decaux

Bruno Colin

Patrick Broyer

Mathieu Dupoy

Denis Desseree

Lorène Allano

Guillaume Sutra

Guillaume Boissier

Lorette Lapierre

Léontine Jacolin

Christophe Tachier

Michel Guy

Jean-Pierre Moy

Frédéric Mallard

Mathieu Debourdeau

Marie-Hélène Charles

Nadine Perrot

Jérôme Blaze

Geneviève Bossy

Régis Montvernay

Loïc Denis

Agnès Dupont Filliard

Nicolas Faure

Magaly Ponsard-Fillette

Thierry Kollaroczy

Agnés Dupont-Fillard

Corinne Fournier

Thomas Olivier

Loréne Allano

Lorette Munoz

Olivier Figadere

Maryse Guicherd

Fabien Romanens

Matthieu Goujard

Agnès Dupont-Fillard

A focus adjustment method for acquiring an image of a surface of interest of a sample by a holographic imager includes the steps of:

Focus adjustment method for holographic imaging system

An extraction system includes a support bar including a sample well, a reagent well, at least one washing well, and at least one protection and retention housing; a pipetting element removably mounted on the support bar and extending at least partially into the at least one protection and retention housing; and a filtering and transfer device configured to capture and accumulate microorganisms contained in the sample and transfer the captured and accumulated microorganisms, the filtering and transfer device being removably mounted on the support bar and extending at least partially into the at least one protection and retention housing. The filtering and transfer device includes a hollow body having an elongated shape and including a proximal end portion and a distal end portion; and a filtering element fixed to the distal end portion of the hollow body.

Extraction system and method for extracting microorganisms contained in a sample

A method for detecting a presence or an absence of at least one zone of inhibition, the method including a step consisting in depositing a volume of the sample in liquid form along a deposition zone extending along an axis at the surface of the agar culture medium and a step consisting in depositing a determined amount of a chemical agent at the surface of the agar culture medium, the deposit defining a potential zone of inhibition, the axis of the zone of deposition of the sample intersecting the potential zone of inhibition.

Method for detecting a presence or absence of at least one first zone of inhibition

A method detects the presence or absence of at least one first inhibition zone having a culture of biological particles on a culture medium in the presence of a chemical agent. The method of detection has a first step of seeding the culture medium with the biological particles, a second step in which the culture medium receives the chemical agent, a third step of incubation of the culture medium, and a fourth step of measurement of the first inhibition zone of the culture medium, which includes a first phase of taking an image of the first inhibition zone by ombroscopy.

Method for detecting the presence or absence of biological particles

A method and system incorporating a process for analyzing a sample to be tested to determine the presence of or to quantify an analyte in the sample. The process employs a reaction which produces a reaction medium derived from the sample that possesses fluorescence properties, in response to illumination by a light source. The process includes illuminating the sample and detecting a fluorescent signal, introducing the reaction medium, and illuminating the sample and detecting a second fluorescent signal from the sample. The process further includes processing the two signals to determine which portion of the fluorescent signal is produced solely by the reaction medium.

Method and system for detecting and measuring fluorescence signals

A locating method and system of determining a microorganism concentration of a fluid in a Petri dish is disclosed. The Petri dish includes a culture medium on which a seeding device is rotatable relative to the Petri dish. The seeding device distributes the fluid and includes at least one point of contact with the culture medium. The point of contact is associated with a contact zone.

Means, method and computer program product for determining the concentration level of microorganisms during a fluid analysis

A method of analyzing a sample to be tested to determine the presence of or to quantify an analyte in the sample, employing a reaction which produces a reaction medium derived from the sample and which possesses fluorescence properties, the reaction medium being located within a well, the reaction medium and the well forming an analysis assembly which possesses fluorescent properties in response to illumination by a light source producing a light signal, the light source being movable along a surface Sof the well.

The present invention relates to a method of sampling biological material on a reference surface, such as the surface of an agar medium, with the aid of a sampling tool, said method making it possible to steer the displacement of the sampling tool towards the reference surface, from a first position towards a second position, and/or of the reference surface towards the sampling tool, from a first position towards a second position, said second position being a contact position in which the sampling tool and the reference surface are in contact, said method comprising the following steps:

Optical method for controlling the movement of a sampling tool

A method for observing biological species on a culture medium contained in a container having at least one translucent face, the method including the steps of: a) directing a light beam onto one portion of the translucent face, so as to define at least one illuminated region and at least one non-illuminated region of the face; and b) acquiring an image of a portion of the surface of the culture medium illuminated by the light beam, the acquisition being carried out through at least one of the non-illuminated regions of the translucent face and along an optical acquisition axis forming a non-zero angle (a) with the direction of propagation of the light beam.

Method for observing biological species

The present invention relates to a disposable device () for amplifying at least one target nucleic acid present in a liquid and biological sample of interest, which consists of a solid body (), at least one fluid channel () connecting an inlet (), via which all or part of the sample of interest can be drawn up and/or discharged, and an outlet (), which is itself connected to a means for the drawing up/discharging of the said sample of interest, the fluid channel () further comprising from the inlet () to the outlet (): The invention also proposes an amplification method using such a device. The said invention has a preferred application in the field of medical diagnosis.

Simplified device for nucleic acid amplification and method for using same

An imaging system for generating images of biological samples having a surface, the system comprising: a sample support for supporting a biological sample in use; a plurality of illumination sources, the plurality of illumination sources being arranged around the sample support and each adapted to illuminate the biological sample, in use, from a different direction; an image capture device for capturing illumination which has impinged on the biological sample to thereby form an image of the sample; wherein at least one of the illumination sources direction is not perpendicular to the surface of the sample.

Imaging method and system for analyzing in-vitro biological samples

A method for defining an isolation area around an object of interest in a cell culture vessel, the method comprising the steps of: &#x2014;obtaining one or more images of the cell culture vessel using one or more of a plurality of illumination sources, each illumination source being capable of illuminating the vessel from a different direction; &#x2014;selecting an image or combination of images for further processing; &#x2014;applying a circular object detection transformation to identify one or more objects of interest being substantially circular objects in the cell culture vessel, which circular objects of interest are representative of isolated colonies in the cell culture vessel and determining the center of an object of interest; &#x2014;applying a binarizing step to obtain a binarized image of the object of interest and other objects, wherein the center of the binarized image corresponds to the center of the object of interest; &#x2014;iteratively forming concentric circles with increasing radius, wherein the concentric circles are centered on the center of the binarized image; &#x2014;identifying coronas, wherein a corona is delimited by two circles having successive radius values; &#x2014;for each corona: determining the presence and the location of any other object located in the corona to determine the presence and the location of other objects; &#x2014;determining a clearance angle defining an angular sector free of other objects around the object of interest to define the isolation area around the object of interest.

Bio-imaging method

A method for enhancing an image of a biological sample, which biological sample has been grown in a vessel on a culture medium.

Bio-imaging method and system

The invention relates to a method for detecting clusters of biological particles () on a surface (), comprising steps that involve: a. determining (E) a topographical representation () of said surface; and b. detecting (E, E), on said topographical representation, at least one contour defining a region that is likely to correspond to a cluster of biological particles.

Method for detecting clusters of biological particles

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Grenoble, France

Frédéric Pinston