Smithkline-rit

Recherche Et Industrie Therapeutiques (r.i.t.)

Smith Kline Rit, S.a.

Frans Van Wijnendaele

Guy Simonet

Constant Huygelen

Lucia Dobrescu

Daniel Gilles

The process is applicable to the supernatant of engineered yeast cells disrupted in the presence of a non-ionic detergent: it comprises the precipitation of contaminants by polyethylene glycol and the treatment cation or, after eventual ultrafiltration, with ammonium sulfate.

Process for the extraction and purification of proteins from culture

The supernatant of engineered yeast cells having produced hepatitis B surface antigaen and disrupted in the presence of a polysorbate non-ionic detergent is clarified by addition of urea and adsorbed on colloidal silica from which the purified antigen is desorbed with a low ionic strength buffer supplemented with urea and a polysorbate non-ionic detergent.

Method for the isolation and purification of hepatitis B surface antigen

Vaccines against oedema disease of piglets comprising an effective amount of E. coli neurotoxin isolated from a culture of an E. coli serotype which is capable of causing piglet oedema disease and adsorbed on an adjuvant. The vaccines are administered by intramuscular or subcutaneous route.

Growing community of inventors

Ottenburg, Belgium

Frans Van Wijnendaele