University of Wyoming

Wyoming Research Products Center

Franco Basile

Erica Belmont

James Rundel

Shaofeng Zhang

Andrew Goodenough

Song Jin

Michael A Urynowicz

David M Bagley

A method and apparatus for monitoring and/or controlling the extent of denaturation and/or bond cleavages of proteins on any surface (e.g., biological tissues, biofilms, etc.). In one embodiment, a low power laser (e.g., a 5 mW, 362 nm diode laser) is directed through a biological sample to a photodetector. The sample is heated by a set of radiant heaters to between about 220° C. and about 250° C. in a time period of between 10 seconds to 60 seconds. The baseline transmissivity of the sample is monitored continuously throughout treatment of the biological sample via continuous monitoring of the signal voltage detected at the photodetector. Upon detection of increase in relative transmissivity in the biological sample, the heating treatment is concluded and the biological sample is removed for in situ protein identification as part of an imaging MALDI-MS measurement.

Non-intrusive laser-based technique for monitor and control of protein denaturation on surfaces

A method and apparatus for monitoring and/or controlling the extent of denaturation and/or bond cleavages of proteins on any surface (e.g., biological tissues, biofilms, etc.). In one embodiment, a low power laser (e.g., a 5 mW, 362 nm diode laser) is directed through a biological sample to a photodetector. The sample is heated by a set of radiant heaters to between about 220&#xb0; C. and about 250&#xb0; C. in a time period of between 10 seconds to 60 seconds. The baseline transmissivity of the sample is monitored continuously throughout treatment of the biological sample via continuous monitoring of the signal voltage detected at the photodetector. Upon detection of increase in relative transmissivity in the biological sample, the heating treatment is concluded and the biological sample is removed for in situ protein identification as part of an imaging MALDI-MS measurement.

A method and apparatus for conducting the rapid pyrolysis of peptides, proteins, polymers, and biological materials. The method can be carried out at atmospheric pressures and takes only about 5 to 30 seconds. The samples are cleaved at the C-terminus of aspartic acid. The apparatus employs a probe on which the sample is heated and digested components analyzed.

Method and apparatus for pyrolysis-induced cleavage in peptides and proteins

A method for converting coal to biogenic methane is provided. The method comprises providing a solid coal matrix, converting the solid coal matrix into soluble, coal-derived constituents, introducing indigenous microorganisms and nutrients into the soluble, coal-derived constituents, and biologically converting the soluble, coal-derived constituents into methane.

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Fort Collins, CO, United States of America

Franco Basile