Centre National De La Recherche Scientifique

Aventis Cropscience S.a.

Rhone-poulenc Agro

Francis Durst

Daniele Werck-Reichhart

Yannick Batard

Michel Schalk

Tiburce Robineau

Luc Didierjean

The present invention relates to a recombinant non-yeast DNA, which encodes a protein of interest, wherein an unmodified DNA corresponding to the recombinant non-yeast DNA contains a region having a high content of codons that are poorly suited to yeasts, wherein a number of the codons that are poorly suited to yeasts are replaced in said region of the recombinant non-yeast DNA with synonymous codons coding for the same amino acid that are well-suited to yeasts, and wherein the number of replaced codons is sufficient to permit expression in yeasts. The present invention also relates to DNA sequences which originate from dicotyledonous or monocotyledonous plants, and in particular plants of the graminae family which are selected from among wheat, barley, oats, rice, maize, sorghum and cane sugar, as well as to vectors and transformed yeasts which contain the DNA sequences of the invention.

Recording of DNA sequences to enable them to be expressed in yeasts, and the transformed yeasts obtained

The present invention relates to the gene CYP76B1 of a cytochrome P450 which has been isolated from tuber. The expression of this gene in yeast has shown that it encoded an enzyme very actively catalysing the O-dealkylation of various exogenous molecules with a high efficiency, such as phenylureas. The expression of CYP76B1 is strongly induced in plants brought into contact with certain exogenous metals or organic compounds. This induction may be exploited for the detection of environmental pollutants. CYP76B1 can be also used to alter the resistance of plants sensitive to this family of herbicides and for soil and groundwater bioremedation.

PURIFIED CYTOCHROME P450 POLYPEPTIDE CYP76B1 FROM HELIANTHUS TUBEROSUS AND ITS APPLICATIONS AS BIOCATALYST IN PARTICULAR FOR THE DEGRADATION OF ENVIRONMENTAL POLLUTANTS AND FOR ALTERING THE RESISTANCE OF PLANTS SENSITIVE TO PHENYLUREA FAMILY OF HERBICIDES

The present invention relates to a DNA sequence which encodes a protein of interest which contains regions having a high content of codons which are poorly suited to yeasts, characterized in that a sufficient number of codons which are poorly suited to yeasts is replaced with corresponding codons which are well-suited to yeasts in the said regions having a high content of codons which are poorly suited to yeasts. The present invention relates, more specifically, to DNA sequences which originate from dicotyledonous or monocotyledonous plants, in particular plants of the graminae family which are selected, in particular, from among wheat, barley, oats, rice, maize, sorghum and cane sugar. The present invention also relates to transformed yeasts which contain a DNA sequence according to the invention.

Recoding of DNA sequences permitting expression in yeast and obtained transformed yeast

Growing community of inventors

Bernolsheim, France

Francis Durst