University of California

A. Menarini Industrie Farmaceutiche Riunite S.r.l.

Ulisse Biomed S.r.l.

Francesco Ricci

Kevin Plaxco

Ryan J White

Hui Li

Netzahualcoyotl Arroyo Curras

Di Kang

Giuseppe Palleschi

Danila Moscone

Alessandro Poscia

Alexis Vallé- Bélisle

Andrew J Bonham

Rudy Ippodrino

Bruna Marini

Alessandro Porchetta

The invention encompasses novel methods of operating electrochemical sensors such as aptamer-based sensors to analyze complex samples, such as flowing whole blood both in vitro or in vivo. In such environments, electrochemical sensors are often subject to drift, which complicates the interpretation of sensor output in terms of target concentration. The method of the invention utilizes a dual-reporter recognition element that generates a first, sensing current that is responsive to target binding and to environmental factors and a second, reference current that is only affected by environmental factors. The reference current provides information about environmentally-induced drift, which allows the drift effect to be subtracted out. By removing drift artifacts, electrochemical sensors may be deployed to analyze complex samples, such as whole blood, in vivo.

Dual-reporter electrochemical sensors with drift correction

Provided are unimolecular oligonucleotide probes for detecting a target in a sample. The probes use target binding-induced structural changes to detect the presence of the target in the sample. Also provided are methods of using the probes to detect a target in a sample.

Nucleotide-based probes and methods for the detection and quantification of macromolecules and other analytes

A process is described for the preparation of modified electrodes useful for the measurement of analytes in biological fluids, comprising the deposition of Prussian blue on screen printed electrodes, and the modified electrodes prepared via said process; the enzymatic electrodes and the biosensors comprising said modified electrodes and the method for the determination of analytes in biological fluids which uses said modified electrodes are also described.

Process for the preparation of modified electrodes, electrodes prepared with said process, and enzymatic biosensors comprising said electrodes

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Rome, Italy

Francesco Ricci