Leland Stanford Junior University

Cetus Corporation

Syva Company

Genelabs, Incorporated

F Carl Grumet

Edgar George Engleman

Steven K Foung

Edwin F Ullman

James W Larrick

Andrew R Raubitschek

Judith A Blunt

Linda B Rabin

A stable trioma cell line capable of secreting a non-human primate monoclonal antibody specific against a selected antigen. An exemplary cell line secretes chimpanzee monoclonal antibody specific against an antigen associated with hepatitis nonA/nonB infection. The cell line is produced, in the method of the invention, by isolating lymphocytes from a primate immunized with the selected antigen, and immortalizing the lymphocytes by fusion with a stable, non-antibody-secreting murine myeloma/human hybridoma cell line having selected-for human characteristics. The trioma fusion products are selected for secretion of the desired antibody, which has a variety of diagnostic and/or therapeutic uses.

Non-human primate monoclonal antibodies and methods

A human monoclonal antibody that directly agglutinates type A human red blood cells is described. The exemplified antibody is an IgM and is produced by hybrid cells lines S-H22 and HHA1. The antibody is useful as an ABO typing reagent.

Human monoclonal antibody against group A red blood cells

By careful screening and mutation, a human-murine hybridoma suitable as a fusion partner for immortalizing an antibody-secreting B cell has been generated. The trioma fusion products of this immortalizing partner are stable producers of human monoclonal antibodies. A trioma which produces monoclonal human anti-varicella zoster is disclosed.

Human-murine hybridoma fusion partner

Improved results are achieved in fluorochromasia lymphocytotoxicity for cell typing by adding antifluorescer to reduce background fluorescence as a result of cell lysing.

Fluorescent determination of microlymphocytotoxicity

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Stanford, CA, United States of America

F Carl Grumet