Sage Science, Inc.

Mosaic Technologies Inc.

Washington University

Exact Sciences Corporation

Matrix Technologies Corporation

First Light Biosciences, Inc.

Boles, T. Christian

Abrams, Ezra S.

Sabin, Douglas Grosvenor

Barbera, Todd J.

Yun, Danny

Ezra Solomon Abrams

T Christian Boles

Todd J Barbera

Douglas Grosvenor Sabin

Philip W Hammond

Andrew R Muir

Danny Yun

Jeffrey D Milbrandt

Stephen Joseph Kron

Sadanand Gite

Don Straus

Robi David Mitra

Gordon Siek

Lisa Shinefeld

Greg Yantz

Tianhong Zhang

Truett C Boles

Brian C Patterson

Slawomir Mielewczyk

A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase reagents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.

Preparative electrophoretic method for targeted purification of genomic DNA fragments

Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.

Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation

The invention provides an improved method for sensitive and specific detection of target molecules, cells, or viruses. The inventive method uses large area imaging to detect individual labeled targets complexed with a target-specific selection moiety. The invention eliminates wash steps through the use of target-specific selection through one or more liquid layers that can contain optical dye and density agents. By eliminating washes the invention simplifies instrumentation engineering and minimizes user steps and costs. The invention uses sensitive image analysis to enumerate individual targets in a large area, is scalable, and can be deployed in systems ranging in complexity from manual to highly automated.

Method for detecting analytes

Systems and methods for processing fluid samples are disclosed. Fluid sample processing is accomplished using a series of microfluidic bump arrays include an automated and integrated system for sorting particles from a biological sample, lysing those particles to expose total RNA or DNA, purifying the RNA or DNA, processing the RNA or DNA by chemical or enzymatic modification, to select RNA or DNA molecules by size, or to generate, optionally, a sequencing library. The sequencing library is suitable for use in next generation sequencing ('NGS').

Systems and methods for processing fluids

Methods of detecting target molecules using electrophoresis and media containing immobilized capture are described.

Electrophoretic analysis of molecules using immobilized probes

A method is provided for immobilizing a ligand, e.g., a nucleic acid, on a solid support. The method includes providing a solid support containing an immobilized latent thiol group, activating the thiol group, contacting the activated thiol group with a nucleic acid comprising an acrylamide functional group, and forming a covalent bond between the two groups, thereby immobilizing the nucleic acid to the solid support. Kits containing the solid supports and method of utilizing the solid supports are also provided.

Methods of immobilizing ligands on solid supports

A multi-stage bridge amplification method which uses a recovered single-stranded amplification single-stranded nucleic acid molecule to initiate second and subsequent stages of bridge amplification is described.

Solid phase methods for amplifying multiple nucleic acids

Methods for determining the presence of a target polynucleotide sequence using chemical hybridization in sequential probe and displacement complex formation with potential for signal gain prior to detection are disclosed.

Multiple sequential polynucleotide displacement reactions for signal amplification and processing

The present invention pertains to a method and compositions for detecting the presence of a target nucleic acid in a test sample in which the sample to be tested for the presence of the target nucleic acid is introduced into a solution containing the probe-tether complex under conditions suitable for hybridization to occur between the first probe nucleic acid sequence and the target nucleic acid sequence so that the second tether nucleic acid sequence is displaced from the probe-tether complex, and a new complex is formed by the hybridization of the first probe nucleic acid sequence and the target nucleic acid sequence

Reverse displacement assay for detection of nucleic acid sequences

Growing community of inventors

Newton, MA, United States of America

Ezra Solomon Abrams