Richter Gedeon Vegyeszeti Gyar Rt

Eva C Pechany

Laszlo Szemler

Maria Langer

Valeria Sike

Gyula Hruby

A fermentation process is disclosed for the preparation of an inoculum, suitable for batchwise, semicontinuous, or continuous production of coenzyme B.sub.12 under septic conditions. The fermentation process begins with a broth consisting of methanol, cornsteep liquor hydrolysate or heat-treated corn slop, ammonium bicarbonate, magnesium chloride, cobalt chloride, 5,6-dimethyl-benzimidazole and sodium bisulfite used for anaerobic, mesophilic, septic fermentation production of coenzyme B.sub.12 with anaerobic, digested sewage sludge. Fermentation is carried out over several steps to provide an inoculum containing an anaerobic, mesophilic, methane-producing, new mixed micropopulation, containing bacteria of each of the types deposited at the Hungarian National Collection of Medical Bacteria, National Institute of Hygiene, under numbers 00076, 00079, and 00272.

Process for the production of inoculum for anaerobic fermentation of

The invention relates to a process for the preparation of a fermentation broth containing coenzyme B.sub.12, with a new, anaerobic, mesophilic, methane-producing mixed micropopulation, under anaerobic, septic conditions, using a new broth containing methanol, precursors and partially known nutrients, In the new process according to the invention the improvement consists in

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Budapest, Hungary

Eva C Pechany