Farmitalia Carlo Erba

Gruppo Lepetit

Ernesto Oppici

Anacleto Gianantonio

Pietro Giardino

Franco Dallatomasina

Onorino G Rosa

Carlo Varesio

Giovanni Lazzari

Roberto Ortica

Danillo Fabris

The invention discloses a method for extracting phenylalanine from the broths obtained by enzymatic processes; this method consists in flocculating and filtering the broth, concentrating the filtrate followed by filtering off the resulting cinnamic acid, decolorating and concentrating the filtrate to obtain phenylalanine crystals at a very pure degree.

Method for extracting phenylalanine from broths of bioconversion

A process for the preparation of the N-L-.alpha.-aspartyl-L-phenylalanine 1-methyl ester (aspartame) which is characterized by adding phosphoric acid and a lower alkyl alcohol to the reaction mixture containing N-formyl .alpha.-L-aspartyl- and .beta.-L-aspartyl-L-phenyl-alanine methyl ester and only one of the resultant deformylated isomers, i.e. aspartame phosphate precipitates. The .alpha.-isomer phosphate is collected by filtration and converted to free aspartame by treatment with a base.

Aspartame synthesis

Disclosed is a process for purifying Tylosin by selective adsorption of the fermentation broth previously brought to 7.5-10.0 pH on polymeric resins and by subsequent deadsorption of the purified product with a aqueous-alcoholic or aqueous-acetonic solution and further purification of the eluate by filtration through macroreticular weak anion resins.

Process for purifying Tylosin

A process for deformylation and separation of N-formyl-L- .alpha.-aspartyl-L-phenylalanine methyl ester from a mixture containing both .alpha. and .beta. isomers thereof, which comprises admixing with said isomeric mixture hydrogen peroxide and an organic acid.

A process for preparing 6-APA and 7-ACA and derivatives therefore is disclosed wherein a penicillin or cephalosporin substrate is reacted with a molar excess of an oxalyl halide of the formula halCOCOhal whereby the COOM group of the substrate, wherein M represents hydrogen, metal or ammonium cations, is transformed into the group COOCOCOhal.

Process for preparing 6-aminopenicillanic acid, 7-aminocephalosporanic

Cephalosporin C is recovered from its fermentation broth by in situ formation of N-(p-nitrobenzoyl)cephalosporin C which is extracted therefrom and converted to 7-amino-cephalosporanic acid.

Process for recovering cephalosporin C from A fermentation broth as the

Process for preparing an .alpha.-aminoalcohol of formula ##EQU1## wherein R represents hydrogen or alkyl of from 1 to 5 carbon atoms, which comprises contacting a compound of formula ##EQU2## wherein R has the same meaning as above, with an alcohol of formula R'-OH wherein R' represents benzyl or the group ##EQU3## in which R' and R'' are independently selected from hydrogen and alkyl of from 1 to 4 carbon atoms in an organic solvent, in the presence of a basic catalyst, at from about 140.degree. to about 170.degree.C., treating the obtained product of formula ##EQU4## wherein R and R' have the above meanings, with at least an equimolecular amount of a p-toluene-sulfonyl halide, at from about -5.degree. to about 20.degree.C. in the presence of a tertiary organic nitrogen-containing base and reacting in a closed system the resulting compound of formula ##EQU5## wherein R and R' are as above defined, with an excess of gaseous ammonia, in the presence of an inert organic solvent, at from about 95.degree. to about 120.degree.C. and recovering the resulting product as its acid addition salt.

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Milan, Italy

Ernesto Oppici