Ajinomoto Co., Ltd.

Ekaterina Sergeevna Preobrazhenskaya

Natalia Viktorovna Stoynova

Tatyana Abramovna Yampolskaya

Vitaliy Arkadievich Livshits

Elena Viktorovna Sycheva

Vsevolod Aleksandrovich Serebryanyy

Lyudmila Anatolievna Kazarinova

Irina Mikhailovna Starovoytova

A mutant bacterial acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine is described. Also described is a method for producing branched-chain L-amino acids using a bacterium from the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by the use of the acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine. This acetolactate synthase contains a mutant small subunit encoded by the mutant ilvN gene.

Mutant acetolactate synthase and a method for producing branched-chain L-amino acids

Uridine-5&prime;-monophosphate is produced by cultivating in a nutrient medium an uridine-5&prime;-monophosphate producing mutant of coryneform bacterium and which is characterized by at least a resistance to growth inhibition by pyrimidine analogue or a deficiency in uridine degrading activity or combination of said property by protoplast fusion. This method has the advantage of decreased production of uracil. Thus, uridine-5&prime;-monophosphate can be produced in much greater yields, compared with known methods.

Method for producing uridine-5'-monophosphate by fermentation using mutant strains of coryneform bacteria

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Moscow, Russia

Ekaterina Sergeevna Preobrazhenskaya