Glycom A/s

Dóra Molnár-Gábor

Gyula Dekany

Pauline Peltier-Pain

Markus Jondelius Hederos

Pierre Chassagne

Markus Hederos

Christophe Pain

Nicolas Fierfort

The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.

Fermentative production of oligosaccharides

The present application discloses a method of making a mixture of 2'-FL and DFL in high yield by culturing, with lactose, a genetically modified cell having a recombinant gene that encodes a single fucosyl transferase. The resulting mixture of 2&#x2032;-FL and DFL can be subjected to hydrolysis initiated by an acid or mediated by a fucosidase to produce fucose in high yields.

Growing community of inventors

Budapest, Hungary

Dóra Molnár-Gábor