Roche Sequencing Solutions, Inc.

Stratos Genomics, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Drew Goodman

Mark Stamatios Kokoris

Aaron Jacobs

Melud Nabavi

Marc Prindle

Samantha Vellucci

John Tabone

Gregory Scott Thiessen

Alexander Isaac Lehmann

John Chase

Jagadeeswaran Chandrasekar

Lacey Merrill

Dylan O'Connell

Jessica Vellucci

Kendall Berg

Dylan O'connell

The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

Enhancement of nucleic acid polymerization by aromatic compounds

Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and accurate incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

DPO4 polymerase variants with improved accuracy

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Seattle, WA, United States of America

Drew Goodman